by Gail Dallalio, Erin Law, and Robert T. Means Hepcidin inhibits in vitro erythroid colony formation at reduced erythropoietin concentrations by Gail Dallalio, Erin Law, and Robert T. Means Blood Volume 107(7):2702-2704 April 1, 2006 ©2006 by American Society of Hematology

Effects of hepcidin on CFU-E on colony formulation. Effects of hepcidin on CFU-E on colony formulation. (A) Effects of hepcidin on CFU-E colony formation at rhEpo 1.0 U/mL. Results are expressed as mean ± SEM; findings from 4 experiments are combined. No values at any hepcidin concentration differ significantly from control (no added hepcidin) or from each other (ANOVA). Mean CFU-E concentration in control clots was 610 per 105 LDMN cells. (B) Effects of 100 ng/mL hepcidin on CFU-E colony formation at reduced rhEpo concentrations are shown. Results are expressed as mean ± SEM; findings from 3 experiments are combined. Colony formation by hepcidin-exposed cells (□, broken line) is significantly less than that of unexposed cells (▪, solid line; P = .031, ANOVA). Mean CFU-E concentration in control clots was 455 per 105 LDMN cells. ACTH(1-24) (100 ng/mL) had no effect on CFU-E colony formation at rhEpo concentrations between 0.1 U/mL and 1.0 U/mL rhEpo. Gail Dallalio et al. Blood 2006;107:2702-2704 ©2006 by American Society of Hematology

Effects of exposure to 100 ng/mL hepcidin on Bad, pBad, and Bcl-xL expression in HCD57 erythroleukemia cells at rhEpo 0.3 U/mL. pBad expression is significantly reduced relative to total Bad or Bcl-xL expression after exposure to hepcidin (P = .02, ANOVA). Effects of exposure to 100 ng/mL hepcidin on Bad, pBad, and Bcl-xL expression in HCD57 erythroleukemia cells at rhEpo 0.3 U/mL. pBad expression is significantly reduced relative to total Bad or Bcl-xL expression after exposure to hepcidin (P = .02, ANOVA). ACTH(1-24) (100 ng/mL) had no effect on pBAD expression by HCD57 cells. Gail Dallalio et al. Blood 2006;107:2702-2704 ©2006 by American Society of Hematology