Volume 8, Issue 7, Pages (July 2015)

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Volume 8, Issue 7, Pages 1115-1118 (July 2015) Yam Tuber Storage Protein Reduces Plant Oxidants Using the Coupled Reactions as Carbonic Anhydrase and Dehydroascorbate Reductase You-Lin Xue, Takuya Miyakawa, Akira Nakamura, Ken-ichi Hatano, Yoriko Sawano, Masaru Tanokura Molecular Plant Volume 8, Issue 7, Pages 1115-1118 (July 2015) DOI: 10.1016/j.molp.2015.02.015 Copyright © 2015 The Author Terms and Conditions

Figure 1 Bioinformatic and Mutational Analyses and Proposed Catalytic Mechanism of Dioscorin. (A) Sequence alignment of dioscorin and NgCA. Important residues for the CA activity of dioscorin are marked with green triangles and cysteine residues for intramolecular disulfide linkage are marked with orange triangles. (B) Superposed structures of dioscorin (magenta) and NgCA (green). The intramolecular disulfide linkage of dioscorin is shown in yellow. (C) Zinc ligands of NgCA (green) and the related residues of dioscorin (magenta). (D) The binding mode of ASC in the CA active site of dioscorin. Hydrogen bonds and a water molecule are represented by yellow dashed lines and a red sphere, respectively. The value along the dashed lines is the bond length (Å) of individual hydrogen bonds. (E and F) DHA reductase (E) and CA (F) activities of the WT and some mutant dioscorins. +Zn indicates the addition of 0.5 mM zinc sulfate into the reaction solution at the final concentration. All determinations were performed in triplicate and expressed as the mean ± SD. (G) Proposed mechanism of DHA reduction coupling with carbon dioxide hydration (shown within the orange rectangle) by dioscorin. Molecular Plant 2015 8, 1115-1118DOI: (10.1016/j.molp.2015.02.015) Copyright © 2015 The Author Terms and Conditions