Masanobu Ishii et al. BTS 2017;2:

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Masanobu Ishii et al. BTS 2017;2:655-668 Beneficial Effects of LCZ696 on Gene Expression 3 Days After MI Real-time reverse transcriptase PCR results for (A) TNFα, (B) IL-1β, (C) IL-6, (D) MCP-1, (E) TGFβ-1, (F) Col1α1, (G) Col3α1, (H) MMP-2, (I) MMP-9, (J) TIMP-1, (K) ANP, and (L) BNP mRNA levels 3 days after MI. The mRNA level was normalized by the level of endogenous control 18S ribosomal RNA. n = 26 per group. In these box-and-whisker plots, lines within the boxes represent median values; the upper and lower lines of the boxes represent the 75th and 25th percentiles, respectively; and the upper and lower bars outside the boxes represent maximum and minimum values within 1.5 times the interquartile range from the 75th and 25th percentile, respectively. *p < 0.05, Bonferroni’s multiple comparison test. ANP = atrial natriuretic peptide; BNP = brain natriuretic peptide; Col = collagen; IL = interleukin; IR = infarcted region; MCP = monocyte chemotactic protein; MMP = matrix metalloproteinase; PCR = polymerase chain reaction; TGF = tumor growth factor; TIMP = tissue inhibitor of metalloproteinase; TNF = tumor necrosis factor; other abbreviation as in Figure 1. Masanobu Ishii et al. BTS 2017;2:655-668 2017 The Authors