Polymerase Chain Reaction

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Research Techniques Made Simple: Polymerase Chain Reaction

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Research Techniques Made Simple: Polymerase Chain Reaction

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Polymerase Chain Reaction Lilit Garibyan, Nidhi Avashia Journal of Investigative Dermatology Volume 133, Issue 3, Pages 1-4 (March 2013) DOI: 10.1038/jid.2013.1 Copyright © 2013 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 1 Schematic presentation of the polymerase chain reaction principle.. Modified from “PCR: Uses, Steps, Purpose,” SchoolWorkHelper, St. Rosemary Educational Institution (http://schoolworkhelper.net/pcr-uses-steps-purpose) Journal of Investigative Dermatology 2013 133, 1-4DOI: (10.1038/jid.2013.1) Copyright © 2013 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 2 Visualization of the PCR products on an agarose gel. Identification of an alternatively spliced mouse Langerin transcript. Ethidium bromide–stained agarose gel showing PCR products from full-length mouse Langerin obtained from C57BL/6 fresh Langerhans cells (fLC) and from fetal skin–derived dendritic cells (FSDDC). From Riedl et al. (2004). Journal of Investigative Dermatology 2013 133, 1-4DOI: (10.1038/jid.2013.1) Copyright © 2013 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 3 Qualitative PCR detects the presence of Merkel cell polyomavirus in cutaneous squamous cell carcinoma in immunocompetent individuals. MCPyV detection. The presence of MCPyV in squamous cell carcinomas, genomic normal DNA, and adjacent skin DNA was determined via PCR using VP1 primers. A representational result is shown, with 6 of 16 samples tested showing a PCR product at 351 bp. All experiments included DNA from an MCPyV plasmid as a positive control (P) and a negative water control (W). M, molecular weight marker; +, positive for virus;, negative for virus. From Dworkin et al. (2009). Journal of Investigative Dermatology 2013 133, 1-4DOI: (10.1038/jid.2013.1) Copyright © 2013 The Society for Investigative Dermatology, Inc Terms and Conditions

Journal of Investigative Dermatology 2013 133, 1-4DOI: (10. 1038/jid Copyright © 2013 The Society for Investigative Dermatology, Inc Terms and Conditions

Journal of Investigative Dermatology 2013 133, 1-4DOI: (10. 1038/jid Copyright © 2013 The Society for Investigative Dermatology, Inc Terms and Conditions