Testicular expression of synaptonemal complex protein 3 (SYCP3) messenger ribonucleic acid in 110 patients with nonobstructive azoospermia Mahmoud Aarabi,

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Testicular expression of synaptonemal complex protein 3 (SYCP3) messenger ribonucleic acid in 110 patients with nonobstructive azoospermia Mahmoud Aarabi, M.D., Mohammad Hossein Modarressi, M.D., Ph.D., Haleh Soltanghoraee, M.D., Reza Behjati, M.D., Naser Amirjannati, M.D., Mohammad Mehdi Akhondi, Ph.D. Fertility and Sterility Volume 86, Issue 2, Pages 325-331 (August 2006) DOI: 10.1016/j.fertnstert.2005.12.070 Copyright © 2006 American Society for Reproductive Medicine Terms and Conditions

FIGURE 1 Sequences of oligonucleotides used in SYCP3 nested PCR. Numbers show the first or second PCR; F = forward; R = reverse. Arabi. Genetics and male infertility. Fertil Steril 2006. Fertility and Sterility 2006 86, 325-331DOI: (10.1016/j.fertnstert.2005.12.070) Copyright © 2006 American Society for Reproductive Medicine Terms and Conditions

FIGURE 2 SYCP3 mRNA expression in azoospermic patients using semiquantitative nested RT-PCR. 0 = no expression; 1+ = mild expression only in second PCR; 2+ = moderate expression in second PCR; 3+ = high expression in second PCR; 4+ = expression in first and second PCR. Arabi. Genetics and male infertility. Fertil Steril 2006. Fertility and Sterility 2006 86, 325-331DOI: (10.1016/j.fertnstert.2005.12.070) Copyright © 2006 American Society for Reproductive Medicine Terms and Conditions

FIGURE 3 (A) Histopathology of testicular samples with hematoxylin and eosin staining (×200). I = Sertoli cell-only syndrome; II = spermatogonial arrest; III = spermatogenesis arrest; IV = hypospermatogenesis. (B) Reverse transcriptase–polymerase chain reaction products of SYCP3 gene in testicular samples on 1.5% agarose gel stained with ethidium bromide. Lanes 1 and 2: patients with no cells or Sertoli cell-only syndrome; lane 3: patients with spermatogonial arrest; lanes 4–7: patients with spermatogenesis arrest; lanes 8 and 9: patients with hypospermatogenesis. PGM1 is used as a positive control; a: PGM1; b: SYCP3 first PCR; c: SYCP3 second PCR; lane 10: negative control (water). Arabi. Genetics and male infertility. Fertil Steril 2006. Fertility and Sterility 2006 86, 325-331DOI: (10.1016/j.fertnstert.2005.12.070) Copyright © 2006 American Society for Reproductive Medicine Terms and Conditions