New intra-renal graft genes associated with tolerance or rejection

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New intra-renal graft genes associated with tolerance or rejection Isabel M. McMorrow, Kavitha Narayan, David H. Sachs, Christian Leguern Kidney International Volume 61, Issue 1, Pages S85-S93 (January 2002) DOI: 10.1046/j.1523-1755.2002.0610s1085.x Copyright © 2002 International Society of Nephrology Terms and Conditions

Figure 1 Schematic of the protocol developed for generation and screening of differential display RT-PCR products. The first level of screening (1) involved an oligo dT primer to select for expressed transcripts, the second (2) depended upon the reproducibility of products in two independent PCR reactions while in the third (3), a RNA slot blot was probed to evaluate the relative expression of each transcript in naïve, tolerant and rejected kidneys. Kidney International 2002 61, S85-S93DOI: (10.1046/j.1523-1755.2002.0610s1085.x) Copyright © 2002 International Society of Nephrology Terms and Conditions

Figure 2 Identification of RA-1. (A) Autoradiograph of DD RT-PCR products amplified from rejected (R) or tolerated (T) renal allografts with same primer set. (B) Slot blot of total RNA from naïve, accepted and rejected renal grafts probed with RA-1 PCR product. Kidney International 2002 61, S85-S93DOI: (10.1046/j.1523-1755.2002.0610s1085.x) Copyright © 2002 International Society of Nephrology Terms and Conditions

Figure 3 Alignment of RA-1 with SPARC. Full length deduced amino acid sequence of RA-1 aligned with bovine, murine, rat and human SPARC. Kidney International 2002 61, S85-S93DOI: (10.1046/j.1523-1755.2002.0610s1085.x) Copyright © 2002 International Society of Nephrology Terms and Conditions

Figure 4 Time course of SPARC expression and creatinine level in:A, B) rejected renal allografts; C, D, E) tolerant renal allografts;F) control animal that underwent a sham renal transplant and was treated with CsA Gtolerant animal that received a second kidney matched to the first in the absence of CsA treatment. SPARC expression (gray bars) was compared to the serum creatinine level (line) as a measure of kidney function and an indirect indicator of rejection. Animal numbers are indicated. Kidney International 2002 61, S85-S93DOI: (10.1046/j.1523-1755.2002.0610s1085.x) Copyright © 2002 International Society of Nephrology Terms and Conditions

Figure 5 Time course of SPARC expression and graft infiltration in a rejected and accepted graft. SPARC expression (black bars) and graft infiltration (gray bars, as measured by CD2) in representative rejected and accepted kidneys. Kidney International 2002 61, S85-S93DOI: (10.1046/j.1523-1755.2002.0610s1085.x) Copyright © 2002 International Society of Nephrology Terms and Conditions