Volume 52, Issue 5, Pages 1365-1373 (November 2007) NeuroD1 Expression in Human Prostate Cancer: Can It Contribute to Neuroendocrine Differentiation Comprehension?  Luca Cindolo, Renato Franco, Monica Cantile, Giulia Schiavo, Giuseppina Liguori, Paolo Chiodini, Liugi Salzano, Riccardo Autorino, Arturo Di Blasi, Mario Falsaperla, Elisa Feudale, Gerardo Botti, Antonio Gallo, Clemente Cillo  European Urology  Volume 52, Issue 5, Pages 1365-1373 (November 2007) DOI: 10.1016/j.eururo.2006.11.030 Copyright © 2006 European Association of Urology Terms and Conditions

Fig. 1 Reverse transcriptase-polymerase chain reaction (RT-PCR) expression of NeuroD1 in (a) EPN (epithelial cell line), EPN+cAMP; PC3 (bone metastasis of prostate cancer), PC3+cAMP; DU145 (brain metastasis of prostate cancer), DU145+cAMP; LNCaP (lymph-node metastasis of prostate cancer), LNCaP+cAMP cells; (b) prostate fibroblasts (PF) and five benign prostate hyperplasia (BPH) biopsies; (c) 11 biopsies of prostate cancers (PC). Control coamplification with a 443-bp β-actin primer is reported. Duplex PCR products were separated by ethidium 1.2% agarose gel electrophoresis. cAMP=cyclic adenosine monophosphate. European Urology 2007 52, 1365-1373DOI: (10.1016/j.eururo.2006.11.030) Copyright © 2006 European Association of Urology Terms and Conditions

Fig. 2 Neuro-D1 immunohistochemical expression. (A) Negative benign prostatic hyperplasia (×40); (B) Negative cancer infiltrating a positive nerve (×63); (C and D) Positive Gleason 3 adenocarcinoma (×40) and detail (×63); (E and F) Focal positivity in Gleason 4 adenocarcinoma (×40) and detail (×63); (G and H) Focal positivity in Gleason 5 combined with Gleason 3 adenocarcinoma (×40) and detail (×63); (I) Focal positivity in adenocarcinoma after antiandrogen therapy (×40); (L) Nuclear and cytoplasmatic positivity in Gleason 5 adenocarcinoma (×63). European Urology 2007 52, 1365-1373DOI: (10.1016/j.eururo.2006.11.030) Copyright © 2006 European Association of Urology Terms and Conditions

Fig. 3 Distribution of chromogranin-A and NeuroD1, synaptophysin, and CD56 positively stained prostate cancer cells according to Gleason sum score. ChrA=chromogranin-A; SNP=synaptophysin; BPH=benign prostatic hyperplasia; pCA=prostate cancer. European Urology 2007 52, 1365-1373DOI: (10.1016/j.eururo.2006.11.030) Copyright © 2006 European Association of Urology Terms and Conditions