Brain-derived neurotrophic factor expression in granulosa lutein cells

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Presentation transcript:

Brain-derived neurotrophic factor expression in granulosa lutein cells Miguel A. Dominguez, Natalie Cho, Bingjun Zhang, Michael S. Neal, Warren G. Foster Reproductive BioMedicine Online Volume 22, Issue 1, Pages 17-24 (January 2011) DOI: 10.1016/j.rbmo.2010.09.001 Copyright © 2010 Reproductive Healthcare Ltd. Terms and Conditions

Figure 1 (A) Brain-derived neurotrophic factor (BDNF) concentration in granulosa lutein cell lysates increased with time in culture. (B) 8-Bromoadenosine cAMP (8Br-cAMP) treatment increased the concentration of BDNF expression in granulosa lutein cells. Each bar is the mean±SEM of three different culture experiments with between two and five replicates for each treatment concentration. Granulosa lutein cells were obtained from three different study subjects and pooled for each experiment. ∗=significant difference from control with P<0.05. Reproductive BioMedicine Online 2011 22, 17-24DOI: (10.1016/j.rbmo.2010.09.001) Copyright © 2010 Reproductive Healthcare Ltd. Terms and Conditions

Figure 2 Increasing concentration of 8Br-cAMP treatment increased the concentration of progesterone in the spent media from granulosa lutein cell cultures. Each bar is the mean±SEM of three different culture experiments with between two and five replicates for each treatment concentration. Granulosa lutein cells were obtained from three different study subjects and pooled for each experiment. Reproductive BioMedicine Online 2011 22, 17-24DOI: (10.1016/j.rbmo.2010.09.001) Copyright © 2010 Reproductive Healthcare Ltd. Terms and Conditions

Figure 3 Representative photomicrographs showing immunostaining for 3β-hydroxysteroid dehydrogenase (3β-HSD), tenascin, brain-derived neurotrophic factor (BDNF) and tyrosine kinase receptor B (TrkB) in mouse ovaries. Bars=100μm. Reproductive BioMedicine Online 2011 22, 17-24DOI: (10.1016/j.rbmo.2010.09.001) Copyright © 2010 Reproductive Healthcare Ltd. Terms and Conditions

Figure 4 Photomicrographs of serial sections of a mouse ovary in di-oestrus showing immunostaining for (A) brain-derived neurotrophic factor (BDNF); (B) tyrosine kinase receptor B (TrkB); (C) 3β-hydroxysteroid dehydrogenase (3β-HSD); (D) tenascin; and (E) negative control. ∗=inactive corpus luteum surrounded on both sides by active corpora lutea which stained more intensely for all four antigens. Bars=50μm. Reproductive BioMedicine Online 2011 22, 17-24DOI: (10.1016/j.rbmo.2010.09.001) Copyright © 2010 Reproductive Healthcare Ltd. Terms and Conditions