Sample name Cell line Primary Ab Secondary Ab Comments

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Presentation transcript:

Sample name Cell line Primary Ab Secondary Ab Comments Permeabilization Code Dilution 080917 - Tube 1 U-PBMC Ab00376 1:100 Anti-rabbit IgG Alexa flour 488 1:1000 Negative control Yes 080917 - Tube 2 500-P211 1:50 Positive control

Sample name Cell line Primary Ab Secondary Ab Comments Permeabilization Code Dilution 080917 - Tube 2 U-PBMC 500-P211 1:50 Anti-rabbit IgG Alexa flour 488 1:1000 Negative control Yes 080917 - Tube 3 T-PBMC Positive control

Sample name Cell line Primary Ab Secondary Ab Comments Permeabilization Code Dilution 080917 - Tube 5 U-PBMC 500-P211Bt 1:50 Streptavidin-PE 1:200 Negative control Yes 080917 - Tube 4 500-P168Bt Streptavidiv-PE Positive control

CONTINUED

CONTINUED

Sample name Cell line Primary Ab Secondary Ab Comments Permeabilization Code Dilution 080917 - Tube 5 U-PBMC 500-P211Bt 1:50 Streptavidin-PE 1:200 Negative control Yes 080917 - Tube 6 T-PBMC Streptavidiv-PE Positive control

Sample name Cell line Primary Ab Secondary Ab Comments Permeabilization Code Dilution 080917 - Tube 1 U93 Goat IgG 1:50 Anti-goat IgG Alexa flour 488 1:200 Negative control Yes 080917 - Tube 2 EB05738-S2G2 Positive control

CONTINUED (U93)