Proteins Biology 12.

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Presentation transcript:

Proteins Biology 12

What is a protein? Structural component of cells and body Hormones Enzymes Transport in blood stream Antibodies

Peptides Monomer of protein – amino acid Polymer of protein – polypeptide Primary  Secondary  Tertiary  Quaternary

Amino Acids Made up of 3 parts 20 amino acids Carboxyl group Amine group R group 20 amino acids 10 non-essential (you can make) 10 essential (MUST eat, you can’t make yourself)

Our Diet Unlike lipids and carbohydrates, we can NOT store protein We must eat each amino acid every day Focus on essential amino acids

How do you build proteins DNA  RNA  amino acid coding  protein

RNA code  Amino Acid Code

Complete Proteins Contains all 10 essential amino acids

How Do You Test for the Presence of Protein? The biuret test is a chemical test used for detecting the presence of peptide bonds. In the presence of peptides, a copper(II) ion forms violet-colored solution the concentration of protein increases proportionately to the intensity of colour Biuret Solution

Data for Protein? How could we record this data? How do I prevent cross contamination? How many trials should I do? What is a positive and negative control?