C. Hussing, M.L. Kampmann, H.S. Mogensen, C. Børsting, N. Morling

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Comparison of techniques for quantification of next-generation sequencing libraries C. Hussing, M.L. Kampmann, H.S. Mogensen, C. Børsting, N. Morling Forensic Science International: Genetics Supplement Series Volume 5, Pages e276-e278 (December 2015) DOI: 10.1016/j.fsigss.2015.09.110 Copyright © 2015 Elsevier Ireland Ltd Terms and Conditions

Fig. 1 Quantification estimates for (A–C) HID-Ion AmpliSeq™ libraries, (D) Sonicated Ion Xpress™ Plus Fragment libraries, and (E) NimbleGen (Roche) capture libraries. Forensic Science International: Genetics Supplement Series 2015 5, e276-e278DOI: (10.1016/j.fsigss.2015.09.110) Copyright © 2015 Elsevier Ireland Ltd Terms and Conditions

Fig. 2 Test of accuracy and sensitivity of various quantification instruments. Dilutions of two dsDNA oligos were used as template, which consisted of the sequences of either the PGM™ (A) or the MiSeq (B) adapters. Measured oligo concentrations were plotted against the oligo concentrations given by the manufacturer of the oligos. Forensic Science International: Genetics Supplement Series 2015 5, e276-e278DOI: (10.1016/j.fsigss.2015.09.110) Copyright © 2015 Elsevier Ireland Ltd Terms and Conditions