FPLC gel‐filtration analysis of RepA–WT and ΔN37.

Slides:

Advertisements

Similar presentations

KM71H pHe7 LAHH3,K1 3P g VLP prep II Bradford assay

Advertisements

KikG structure (Protein Data Bank accession code 1XSS).

Hydration profiles of GLIC′s pore in WT and Ser6′ mutant simulations.

Sequence and expression of Xenopus EAF2.

Mba1 cofractionates with mitochondrial ribosomes on sucrose gradients.

Vera L. Valente-Mesquita, Michelle M. Botelho, Sérgio T. Ferreira

Assembly of MtGimα and MtGimβ into a hexameric complex with high α‐helical content. Assembly of MtGimα and MtGimβ into a hexameric complex with high α‐helical.

Colocalization of the human trimethylguanosine synthase 1 and survival of motor neuron proteins. Colocalization of the human trimethylguanosine synthase.

DNA‐anchored LacI–CFP–Luciferase aggregates capture misfolded cytosolic proteins. DNA‐anchored LacI–CFP–Luciferase aggregates capture misfolded cytosolic.

Transcribed strand breaks do not interfere with TFIIIB‐dependent recruitment of pol III to the promoter. Transcribed strand breaks do not interfere with.

(A, B) Stereoscopic views of the P3 RNase MRP RNA domain in complex with the RNase MRP/RNase P protein components Pop6 and Pop7. (A, B) Stereoscopic views.

Binding of APC‐derived C‐terminal peptides to EB1‐C.

Yeast Tgl3p expressed in HeLa cells localizes to lipid droplets.

Face‐to‐face kinase domain dimerisation of human Ire1α.

Cytochrome c and ATP hydrolysis are required for Apaf‐1 to interact with procaspase‐9. Cytochrome c and ATP hydrolysis are required for Apaf‐1 to interact.

AO domain of LSD1 is responsible for its interaction with Snail1.

Schematic representations of the sequestering and capping of actin by thymosin, capping protein, gelsolin and formins. Schematic representations of the.

Data analysis of thermal proteome profiling

ERT and uncoating assay shows increased vector capsid uncoating upon successful central DNA Flap formation. ERT and uncoating assay shows increased vector.

Recognition of double‐stranded telomeric DNA

SPR and NMR analyses of interactions of HOIL‐1L–UBL with the HOIP–UBA derivative. SPR and NMR analyses of interactions of HOIL‐1L–UBL with the HOIP–UBA.

Liquid droplet characteristics of p‐tau441

Non‐specific activation of gene transcription by exogenous Fe65 in luciferase‐based reporter assays. Non‐specific activation of gene transcription by exogenous.

Stoichiometry of TGF‐β receptor complexes determined by HPLC analysis.

Circadian gene expression induced by prostaglandin E2 (PGE2) in NIH3T3 cells. Circadian gene expression induced by prostaglandin E2 (PGE2) in NIH3T3 cells.

Proteins with hotspot mutations are often in the interaction networks with known cancer driver proteins Proteins with hotspot mutations are often in the.

Structure‐based functional characterization of H

Validation of the CCE associations by mass spectrometry.

Volume 15, Issue 6, Pages (September 2004)

by Xingwei Sui, Sanford B. Krantz, and Zhizhuang Zhao

Tertiary structure characterization with 1H‐NMR at 900 MHz

Structural Basis for Vertebrate Filamin Dimerization

Volume 15, Issue 6, Pages (June 2007)

The chloroplast 4.5S ribosomal RNA

Direct Binding of Cholesterol to the Purified Membrane Region of SCAP

Jai A. Pathak, Rumi R. Sologuren, Rojaramani Narwal

Usp5 sensitivity of S65 ubiquitin mutant chains Structure of ubiquitin in complex with Usp5 (PDB ID: 3IHP). Usp5 sensitivity of S65 ubiquitin mutant chains.

The MSP domain of MOSPD2 binds the FFAT motif with an affinity in the micromolar range The MSP domain of MOSPD2 binds the FFAT motif with an affinity in.

Volume 26, Issue 2, Pages e3 (February 2018)

Example of processed data visualized with “Peak Browser” of ChIP‐Atlas

Structure and function of mutationally generated monomers of dimeric phosphoribosylanthranilate isomerase from Thermotoga maritima Ralf Thoma, Michael.

XLF Regulates Filament Architecture of the XRCC4·Ligase IV Complex

Structural Basis for Vertebrate Filamin Dimerization

Volume 10, Issue 5, Pages (May 2002)

Engineered Domain Swapping as an On/Off Switch for Protein Function

Whole‐mount in situ hybridization analysis of marker genes in wild‐type embryos and chokh mutants. Whole‐mount in situ hybridization analysis of marker.

Resolution and quality of the cryo‐EM reconstructions

Comparison of nuclear surface area of HeLa and RKO cells

CUL3Δ403–459 displays increased auto‐ubiquitylation and ubiquitylation of KLHL3 A–DIn vitro ubiquitylation assays with purified proteins. CUL3Δ403–459.

CD44 binding surface of the Tiam2 PHCCEx domain.

Scarlet S. Shell, Christopher D. Putnam, Richard D. Kolodner

Volume 96, Issue 8, Pages (April 2009)

Characterising dermis expansion and gene expression changes during mouse development (related to Fig 1)‏ Characterising dermis expansion and gene expression.

Rescue of motor neurons from death The ventral root of the fifth lumbar segment (L5). Rescue of motor neurons from death The ventral root of the fifth.

Volume 17, Issue 8, Pages (August 2009)

PRC2 and HOTAIR in vitro production

In vitro interaction studies (related to Fig 2)‏

Volume 100, Issue 8, Pages (April 2011)

Sequence dependence of Met4 recruitment.

Volume 23, Issue 2, Pages (July 2006)

dsRBDs 3 and 4 of hStau1 dimerize on the RNA.

Rogert Bauer, Rita Carrotta, Christian Rischel, Lars Øgendal

Volume 23, Issue 1, Pages (January 2015)

The AXH Domain Adopts Alternative Folds

Biochemical analysis of γ-TuRC components.

Jochen Zimmer, Declan A. Doyle, J. Günter Grossmann

Volume 13, Issue 3, Pages (February 2004)

XLF Regulates Filament Architecture of the XRCC4·Ligase IV Complex

Reverse-Chaperoning Activity of an AAA+ Protein

The Structure of the MAP2K MEK6 Reveals an Autoinhibitory Dimer

Presentation transcript:

FPLC gel‐filtration analysis of RepA–WT and ΔN37. FPLC gel‐filtration analysis of RepA–WT and ΔN37. Protein samples in storage buffer were run at 0.4 ml/min through a Superose‐12 HR‐10/30 column at room temperature. Elution profiles were obtained monitoring A280 of the eluate. Insets to the panels indicate the sample identity, the concentration at injection and the species assigned to the observed peaks: monomers (wide and irregular), dimers (narrow and sharp) or larger aggregates. The estimated Rs and a cartoon with the proposed shapes (compact and spherical versus flexible and extended) for the proteins in the main peaks are also shown (colour coding for the domains is as in Figure 9). Rafael Giraldo et al. EMBO J. 1998;17:4511-4526 © as stated in the article, figure or figure legend