Odelya E. Pagovich, MD, Bo Wang, MD, Maria J

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Presentation transcript:

Anti-hIgE gene therapy of peanut-induced anaphylaxis in a humanized murine model of peanut allergy Odelya E. Pagovich, MD, Bo Wang, MD, Maria J. Chiuchiolo, PhD, Stephen M. Kaminsky, PhD, Dolan Sondhi, PhD, Clarisse L. Jose, BS, Christina C. Price, MD, Sarah F. Brooks, BSc, Jason G. Mezey, PhD, Ronald G. Crystal, MD Journal of Allergy and Clinical Immunology Volume 138, Issue 6, Pages 1652-1662.e7 (December 2016) DOI: 10.1016/j.jaci.2016.03.053 Copyright © 2016 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Journal of Allergy and Clinical Immunology 2016 138, 1652-1662 Journal of Allergy and Clinical Immunology 2016 138, 1652-1662.e7DOI: (10.1016/j.jaci.2016.03.053) Copyright © 2016 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 1 Characterization of NSG mice reconstituted with human blood mononuclear cells from a donor with peanut (PN) allergy and a control donor. The example shown is with reconstitution from a donor with peanut allergy (PN01) compared with the control donor (C01; see Table E1). A, Timeline of reconstitution, peanut sensitization, and challenge, with interventions indicated by arrows. B, Human IgG levels (ELISA). C, Total human IgE levels (ELISA). D, Peanut-specific IgE (ELISA). E, Examples of appearance of mice after peanut extract challenge at week 5. Left, Mouse reconstituted with mononuclear cells from a nonallergic donor appears normal after peanut challenge. Right, Mouse reconstituted with mononuclear cells from a donor with peanut allergy displaying puffiness around the eyes/snout, pilar erecti, and itching/ruffling of fur 1 minute after peanut challenge. F, Anaphylaxis score (1-5) 30 minutes after challenge at week 5: mouse donor with no peanut allergy and mouse donor with peanut allergy. G, Plasma histamine levels 30 minutes after peanut challenge at week 6: mouse donor with no peanut allergy and mouse donor with peanut allergy. In Fig 1, B-D, F, G, all data are expressed as means ± SEMs. H, PCA. Shown is extravasation of blue dye using human serum from a patient with peanut allergy and serum from a subject without peanut allergy. Journal of Allergy and Clinical Immunology 2016 138, 1652-1662.e7DOI: (10.1016/j.jaci.2016.03.053) Copyright © 2016 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 2 Treatment of peanut (PN) antigen–induced anaphylaxis with omalizumab after sensitization and peanut challenge. The examples shown are from donors PN01 and PN02. A, Free IgE levels quantified by means of ELISA 1 week before and 1 week after therapy with omalizumab (250 μg). Data are expressed as means ± SEMs. B, Mice after peanut extract challenge. Shown are data 2 weeks after therapy. The mouse treated with omalizumab appeared normal after peanut challenge (compare with Fig 1, E). C, Omalizumab-mediated suppression of PCA. Omalizumab blocked peanut-induced, peanut-specific IgE-mediated PCA (compare with Fig 1, H). Journal of Allergy and Clinical Immunology 2016 138, 1652-1662.e7DOI: (10.1016/j.jaci.2016.03.053) Copyright © 2016 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 3 Characterization of AAVrh.10anti-hIgE. A, Schematic with CMV enhancer/chicken β-actin (CAG) promoter, heavy and light chains of the anti-hIgE mAb omalizumab, furin 2A cleavage site, and polyadenylation signal. B, In vitro pAAV-anti-hIgE directed expression of omalizumab in 293HEK cells. C, Persistence of expression of the anti-hIgE antibody over time after a single intravenous administration of AAVrh.10anti-hIgE (1011 genome copies) to NSG mice. AAVrh.10IgGcontrol (1011 genome copies) and PBS were controls. Values are presented as means ± SEMs. Journal of Allergy and Clinical Immunology 2016 138, 1652-1662.e7DOI: (10.1016/j.jaci.2016.03.053) Copyright © 2016 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 4 Prophylaxis of peanut (PN) antigen–induced anaphylaxis by treatment with AAVrh.10anti-hIgE before sensitization. A, Prophylaxis timeline of reconstitution, peanut sensitization, and challenge, with interventions indicated by arrows. The examples shown are from donors PN01 and PN03 with peanut allergy and control donor C01 (see Table E1). B, Total human IgE evaluated after completion of peanut sensitization protocol (week 4). C, Total peanut specific IgE after completion of peanut sensitization protocol (week 4). D, Free IgE levels evaluated by using ELISA at week 4. Data in Fig 4, B-D, are expressed as means ± SEMs. Journal of Allergy and Clinical Immunology 2016 138, 1652-1662.e7DOI: (10.1016/j.jaci.2016.03.053) Copyright © 2016 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 5 Prophylaxis of peanut (PN) antigen–induced anaphylaxis by prior treatment with AAVrh.10anti-hIgE. A, Mice after peanut extract challenge at week 6. Left, Mouse treated with a control vector at week −3 displayed puffiness around the eyes/snout and pilar erecti, itching/ruffling of fur, and decreased ambulation and respiratory rate after peanut challenge. Right, Mouse treated with AAVrh.10anti-hIgE at week −3 appeared normal after peanut challenge. B, Locomotor activity. Shown are data of the distance traversed over 30 minutes after peanut challenge in vector- and control-treated mice assessed at week 6. C, Anaphylaxis score 30 minutes after peanut challenge at week 6. D, Plasma histamine levels 30 minutes after peanut challenge at week 7. Data in Fig 5, B-D, are expressed as means ± SEMs. E, AAVrh.10anti-hIgE–mediated suppression of PCA. Left, Peanut extract–induced PCA mediated by peanut-specific IgE from the serum of a donor with peanut allergy. Right, Peanut extract induced PCA-mediated by peanut-specific IgE from the pooled serum of the humanized NSG mice with peanut allergy reconstituted by using the same donor as in the left panel but treated prophylactically with AAVrh.10anti-hIgE at week −3 before sensitization. The sera from the AAVrh.10anti-hIgE–treated mice blocked peanut-induced, peanut-specific IgE-mediated PCA compared with the AAVrh.10IgGcontrol. Journal of Allergy and Clinical Immunology 2016 138, 1652-1662.e7DOI: (10.1016/j.jaci.2016.03.053) Copyright © 2016 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 6 Treatment of peanut (PN) antigen–induced anaphylaxis with AAVrh.10-anti-hIgE or omalizumab after mice exhibited peanut-specific allergic symptoms after sensitization and peanut challenge. A, Therapeutic timeline of reconstitution, peanut sensitization, and challenge, with interventions indicated by arrows. Examples shown in Fig 6, B-D, are from donors PN01 and PN02. B, Total human IgE evaluated by means of ELISA (week 4, 1 week before therapy; week 6, 1 week after therapy). C, Total peanut-specific IgE evaluated by means of ELISA at week 4, 1 week before therapy. D, Free IgE evaluated by means of ELISA at week 4, 1 week before therapy, and week 6, 1 week after therapy. Data in Fig 6, B-D, are expressed as means ± SEMs. Journal of Allergy and Clinical Immunology 2016 138, 1652-1662.e7DOI: (10.1016/j.jaci.2016.03.053) Copyright © 2016 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 7 Treatment of peanut (PN) antigen–induced anaphylaxis with AAVrh.10-anti-hIgE compared with omalizumab after sensitization and peanut challenge. A, Mice after peanut extract challenge. Shown are data for week 10, 5 weeks after therapy. Left, Mouse treated with omalizumab (week 5) displayed puffiness around the eyes/snout and pilar erecti, itching/ruffling of fur, and decreased ambulation and respiratory rate after peanut challenge (compare with Fig 2, B: mouse 2 weeks after treatment with omalizumab). Right, Mouse treated 5 weeks previously with AAVrh.10anti-hIgE appeared normal after peanut challenge. B, Locomotor activity. Shown are data of the distance traversed over 30 minutes after peanut challenge for week 7, 2 weeks after therapy, and week 10, 5 weeks after therapy. C, Anaphylaxis score (1-5) 30 minutes after peanut challenge. Shown are data for week 7, 2 weeks after therapy, and week 10, 5 weeks after therapy. D, Plasma histamine levels 30 minutes after peanut challenge. Shown are data for week 6, 1 week after therapy, and week 9, 4 weeks after therapy. Only 1 AAVrh.10IgG control-treated mouse was alive at weeks 9 and 10, and therefore statistical analysis was not included. Data for Fig 7, B-D, expressed as means ± SEMs. E, AAVrh.10anti-hIgE–mediated suppression of PCA. Upper panel, Peanut extract–induced PCA mediated by peanut-specific IgE from serum of a donor with peanut allergy but not from a nonallergic control donor. Lower panel, Week 10, 5 weeks after therapy. Persistent expression of AAVrh.10anti-hIgE blocks extravasation of dye, whereas a 1-time injection of omalizumab 5 weeks previously was no longer protective. Omalizumab blocked peanut-induced, peanut-specific IgE-mediated PCA 2 weeks after omalizumab therapy (Fig 2, C). Journal of Allergy and Clinical Immunology 2016 138, 1652-1662.e7DOI: (10.1016/j.jaci.2016.03.053) Copyright © 2016 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 8 Mouse survival after treatment with a single administration of AAVrh.10anti-hIgE, single administration of omalizumab alone, or control vector. Survival duration and treatment type are shown days after therapy. Journal of Allergy and Clinical Immunology 2016 138, 1652-1662.e7DOI: (10.1016/j.jaci.2016.03.053) Copyright © 2016 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig E1 Major cellular interactions during the mucosal allergic immune response and therapy with anti-hIgE. Sensitization: In the gastric mucosa peanut allergens are taken up by M cells and transferred to antigen-presenting cells (APC), where they are processed into peptide fragments. Complexes of peanut-allergen peptide and major histocompatibility complex (MHC II) are presented to naive T cells, which differentiate into TH2 cells. Activated TH2 cells recognize peanut allergen peptide–MHC complexes on the surfaces of B cells, releasing the cytokines IL-4 and IL-13, which promote IgE antibody production by B cells. Secreted IgE antibodies bind to FcεRI receptors on effector cells, such as mast cells, which become sensitized. Exposure to peanuts: On secondary encounter with the same peanut allergen, the allergen crosslinks cell-bound IgE, activating mast cells to release inflammatory mediators. Additional production of IL-4 and IL-13 by mast cells and basophils results in further TH2 cell differentiation and IgE synthesis. This cascade of events ultimately leads to induction of symptoms commonly associated with peanut allergy. Therapy with anti-hIgE: Anti-hIgE binds to the third constant domain of the IgE heavy chain (Cε3), the same site at which IgE normally binds to both high- and low-affinity IgE receptors on mast cells, basophils, and other cell types. Anti-hIgE forms complexes with free IgE and prevents its interaction with these receptors. Journal of Allergy and Clinical Immunology 2016 138, 1652-1662.e7DOI: (10.1016/j.jaci.2016.03.053) Copyright © 2016 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig E2 SDS-PAGE of peanut allergens. Arrows indicate the known peanut extract proteins Ara h1, Ara h2, Ara h3, and Ara h4.E9 Journal of Allergy and Clinical Immunology 2016 138, 1652-1662.e7DOI: (10.1016/j.jaci.2016.03.053) Copyright © 2016 American Academy of Allergy, Asthma & Immunology Terms and Conditions