Volume 76, Issue 4, Pages 395-403 (August 2009) Kallikrein protects against microalbuminuria in experimental type I diabetes  Sophie Bodin, Catherine Chollet, Nicolas Goncalves-Mendes, Joelle Gardes, Franck Pean, Didier Heudes, Patrick Bruneval, Michel Marre, François Alhenc-Gelas, Nadine Bouby  Kidney International  Volume 76, Issue 4, Pages 395-403 (August 2009) DOI: 10.1038/ki.2009.208 Copyright © 2009 International Society of Nephrology Terms and Conditions

Figure 1 Urinary albumin excretion in wild-type (WT) and tissue kallikrein–deficient (TK-null) mice 1 and 2 months after induction of diabetes (series B). Black bars: diabetic groups, white bars: control groups. Results are means±s.e.m. n=7–8/group. Analysis of variance followed by Fisher PLSD post hoc test, diabetic vs control: ***P<0.001; TK-null vs WT: ##P<0.01. Kidney International 2009 76, 395-403DOI: (10.1038/ki.2009.208) Copyright © 2009 International Society of Nephrology Terms and Conditions

Figure 2 Systolic blood pressure in wild-type (WT) and tissue kallikrein–deficient (TK-null) mice 1 and 2 months after induction of diabetes (series B). Black bars: diabetic groups, white bars: control groups. Results are means±s.e.m. n=7–8/group. Analysis of variance followed by Fisher PLSD post hoc test, diabetic vs control: ***P<0.001, TK-null vs WT: nonsignificant. Kidney International 2009 76, 395-403DOI: (10.1038/ki.2009.208) Copyright © 2009 International Society of Nephrology Terms and Conditions

Figure 3 Gene expression of the components of the renal kallikrein–kinin system in wild-type (WT) and tissue kallikrein–deficient (TK-null) mice 1 month after induction of diabetes (series A). Black bars: diabetic groups, white bars: control groups. Results are means±s.e.m. n=10–12/group. Results for each gene are expressed relative to the mean of the corresponding WT control group. Analysis of variance followed by Fisher PLSD post hoc test, diabetic vs control: ***P<0.0001, **P<0.01, TK-null vs WT: nonsignificant. Kidney International 2009 76, 395-403DOI: (10.1038/ki.2009.208) Copyright © 2009 International Society of Nephrology Terms and Conditions

Figure 4 Gene expression of ACE in kidney and lung of wild-type (WT) and tissue kallikrein–deficient (TK-null) mice 1 month after induction of diabetes (series A). Black bars: diabetic groups, white bars: control groups. Results are means±s.e.m. n=10–12/group. Results for each gene are expressed relative to the mean of the corresponding WT control group. Analysis of variance followed by Fisher PLSD post hoc test, diabetic vs control: ***P<0.0001, TK-null vs WT: nonsignificant. ACE, angiotensin I–converting enzyme. Kidney International 2009 76, 395-403DOI: (10.1038/ki.2009.208) Copyright © 2009 International Society of Nephrology Terms and Conditions

Figure 5 Gene expression of the components of the renal renin–angiotensin system in wild-type (WT) and tissue kallikrein–deficient (TK-null) mice 1 month after induction of diabetes (series A). Black bars: diabetic groups, white bars: control groups. Results are means±s.e.m. n=10–12/group. Results for each gene are expressed relative to the mean of the corresponding WT control group. Analysis of variance followed by Fisher PLSD post hoc test, diabetic vs control: ***P<0.0001, *P<0.05, TK-null vs WT; #P<0.05. Kidney International 2009 76, 395-403DOI: (10.1038/ki.2009.208) Copyright © 2009 International Society of Nephrology Terms and Conditions

Figure 6 Gene expression of precursors of the kallikrein–kinin and renin–angiotensin system in the liver of wild-type (WT) and tissue kallikrein–deficient (TK-null) mice 1 month after induction of diabetes (series A). Black bars: diabetic groups, white bars: control groups. Results are means±s.e.m. n=10–12/group. Results for each gene are expressed relative to the mean of the corresponding WT control group. Analysis of variance followed by Fisher PLSD post hoc test, diabetic vs control: ***P<0.0001, *P<0.05, TK-null vs WT; nonsignificant. Kidney International 2009 76, 395-403DOI: (10.1038/ki.2009.208) Copyright © 2009 International Society of Nephrology Terms and Conditions