Gloria García-Casado, PhD, Jesús F

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A novel wheat gliadin as a cause of exercise-induced anaphylaxis

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Presentation transcript:

Isolation and characterization of barley lipid transfer protein and protein Z as beer allergens Gloria García-Casado, PhD, Jesús F. Crespo, MD, PhD, Julia Rodríguez, MD, Gabriel Salcedo, PhD Journal of Allergy and Clinical Immunology Volume 108, Issue 4, Pages 647-649 (October 2001) DOI: 10.1067/mai.2001.118793 Copyright © 2001 Mosby, Inc. Terms and Conditions

Fig. 1 SDS-PAGE fractionation of a crude protein preparation (lane 1 ; 15 μg of protein) and of purified 9-kd (lane 2 ) and 45-kd (lane 3 ; 3 μg) proteins from beer, stained with Coomassie blue (A ) or electrotransferred to nitrocellulose membrane and immunoblotted with a serum pool from beer-allergic patients (B ). C, IgE immunodetection after dot-blotting (3 μg of protein) a crude protein preparation from beer (T) , and purified barley LTP1 (L) and protein Z4 (Z) . Bovine serum albumin (B) and PBS buffer (N) were used as negative controls. Four individual sera (1-4; numbers correspond to those in Table I) from selected beer-allergic patients were used. Journal of Allergy and Clinical Immunology 2001 108, 647-649DOI: (10.1067/mai.2001.118793) Copyright © 2001 Mosby, Inc. Terms and Conditions