Strong FANCA/FANCG but weak FANCA/FANCC interaction in the yeast 2-hybrid system by Tanja Reuter, Sabine Herterich, Oliver Bernhard, Holger Hoehn, and.

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Strong FANCA/FANCG but weak FANCA/FANCC interaction in the yeast 2-hybrid system by Tanja Reuter, Sabine Herterich, Oliver Bernhard, Holger Hoehn, and Hans J. Gross Blood Volume 95(2):719-720 January 15, 2000 ©2000 by American Society of Hematology

Yeast plates showing growth response under different dilution and testing conditions.Yeast strain EGY48 was cotransfected in pairs, with the indicated combinations of vectors for the expression of full-length Fanconi anemia LexA or B42 fusion proteins. Yeast plates showing growth response under different dilution and testing conditions.Yeast strain EGY48 was cotransfected in pairs, with the indicated combinations of vectors for the expression of full-length Fanconi anemia LexA or B42 fusion proteins. Three dilutions each were spotted onto complete minimal dropout media. The first protein of each of the combinations represents the LexA fusion, the second protein the B42 fusion. Each result was confirmed by 4 independent assays. Tanja Reuter et al. Blood 2000;95:719-720 ©2000 by American Society of Hematology