Cell composition of the human pulmonary valve: a comparative study with the aortic valve–the VESALIO∗ project∗ Foscarina Della Rocca, MD, Saverio Sartore,

Slides:

Advertisements

Similar presentations

Aortic Valve Replacement With Patch Enlargement of the Aortic Annulus

Advertisements

Uswa Shahzad, BHSc, Guangming Li, MD, Yaoguang Zhang, MD, Terrence M

Max B. Mitchell, MD The Journal of Thoracic and Cardiovascular Surgery

Evidence for an altered balance between matrix metalloproteinase-9 and its inhibitors in calcific aortic stenosis Jari Satta, MD, PhD, Jani Oiva, MS,

Impact of Cryopreservation on Extracellular Matrix Structures of Heart Valve Leaflets Katja Schenke-Layland, PhD, Navid Madershahian, MD, Iris Riemann,

Henna M. Karvonen, MSc, Siri T. Lehtonen, PhD, Raija T

Hemolytic Anemia With Aortic Stenosis Resolved by Urgent Aortic Valve Replacement Isamu Kawase, MD, Tatsuro Matsuo, MD, Koji Sasayama, CCP, Hiroyuki Suzuki,

Analysis of Osteopontin Levels for the Identification of Asymptomatic Patients With Calcific Aortic Valve Disease Juan B. Grau, MD, Paolo Poggio, MS,

Differential proteome analysis of normal and osteoarthritic chondrocytes reveals distortion of vimentin network in osteoarthritis S. Lambrecht, M.Pharm.,

Robert L. Smith, MD, Morley A. Herbert, PhD, Todd M

Pressure distention compared with pharmacologic relaxation in vein grafting upregulates matrix metalloproteinase-2 and -9 Ada W.Y. Chung, PhD, Pooja.

Fluorescence activated cell sorting: A reliable method in tissue engineering of a bioprosthetic heart valve Simon P Hoerstrup, MD, Gregor Zünd, MD, Andreina.

Volume 117, Issue 5, Pages (November 1999)

Continual Exposure to Cigarette Smoke Extracts Induces Tumor-Like Transformation of Human Nontumor Bronchial Epithelial Cells in a Microfluidic Chip

Heat shock protein (HSP 72) expression in patients undergoing cardiac operations Lynn B. McGrath, MD, Marius Locke, PhD, Michael Cane, MD, Chao Chen,

Immunology and failure of valved allografts in children

Cell characterization of porcine aortic valve and decellularized leaflets repopulated with aortic valve interstitial cells: the VESALIO project (vitalitate.

Long-term results of surgical valvuloplasty for congenital valvar aortic stenosis in children Claude C Chartrand, MD, Enrique Saro-Servando, MD, Jitka.

Severe Pulmonary Valve Regurgitation 40 Years After Blunt Chest Trauma

Peripheral detection of S100β during cardiothoracic surgery: what are we really measuring? Vincent Fazio, MS, Sunil K Bhudia, MD, Nicola Marchi, PhD,

Ascending aorta dilation in association with bicuspid aortic valve: A maturation defect of the aortic wall Nimrat Grewal, MD, Adriana C. Gittenberger-de.

CTSNet: a unique educational vehicle

Volume 18, Issue 4, Pages (April 2016)

Ioannis K. Toumpoulis, MD, Julia Thom Oxford, PhD, Douglas B

Transforming Growth Factor-β1 Mechanisms in Aortic Valve Calcification: Increased Alkaline Phosphatase and Related Events Jocelyn N. Clark-Greuel, PhD,

Inhibitory effect of photooxidation on intimal and medial thickening of saphenous vein Jyotirmay Chanda , MD, Ryosei Kuribayashi, MD, Ke-Xiang Liu, MD,

Avrum I. Gotlieb, MD, CM, Alan Rosenthal, PhD, Pedram Kazemian, BSc

Dynamic alterations in the expression and localization of ACTL7a during capacitation in mouse spermatozoa Jun Fu, Ph.D., Wei Song, Ph.D., Shudong Zong,

Michael A. Golden, MD, Y. P. Tina Au, PhD, Richard D

The extent of degeneration of cruciate ligament is associated with chondrogenic differentiation in patients with osteoarthritis of the knee K. Kumagai,

Recombinant human hepatocyte growth factor transfection alleviates hyperkinetic pulmonary artery hypertension in rabbit models Wei Wang, MD, Kai Liu,

Aortic valve cusp vessel density: Relationship with tissue thickness

Protection by 20-5,14-HEDGE Against Surgically Induced Ischemia Reperfusion Lung Injury in Rats Irshad Ali, PhD, Stephanie Gruenloh, BS, Ying Gao, MS,

Mark F Berry, MD, Y.Joseph Woo, MD The Annals of Thoracic Surgery

Expression of Nuclear Factor-κB and Its Clinical Significance in Nonsmall-Cell Lung Cancer Zhenfa Zhang, MD, Jianqun Ma, MD, Ni Li, PhD, Nan Sun, MD,

Stapling of an aortic arch aneurysm

High Invasive Melanoma Cells Induce Matrix Metalloproteinase-1 Synthesis in Fibroblasts by Interleukin-1α and Basic Fibroblast Growth Factor-Mediated.

Continual Exposure to Cigarette Smoke Extracts Induces Tumor-Like Transformation of Human Nontumor Bronchial Epithelial Cells in a Microfluidic Chip

Inhibition of ADAMTS-7 and ADAMTS-12 degradation of cartilage oligomeric matrix protein by alpha-2-macroglobulin Y. Luan, Ph.D., M.D., L. Kong, Ph.D.,

Optimized Preservation of Extracellular Matrix in Cardiac Tissues: Implications for Long- Term Graft Durability Katja Schenke-Layland, PhD, Jiansong Xie,

Kazunori Yoshida, MD, Satoshi Tobe, MD, Masahito Kawata, MD

Evidence for an altered balance between matrix metalloproteinase-9 and its inhibitors in calcific aortic stenosis Jari Satta, MD, PhD, Jani Oiva, MS,

Dynamic analysis of the aortic valve using a finite element model

Michele Gallo, MD, Gino Gerosa, MD

Spindle Cell Sarcoma of the Mitral Valve: An Unusual Cause of Acute Coronary Syndrome in a Child Thomas Martens, MD, Kristof Vandekerckhove, MD, Katrien.

Significant improvement of heart function by cotransplantation of human mesenchymal stem cells and fetal cardiomyocytes in postinfarcted pigs Jiang-Yong.

Mechanical Strain and the Aortic Valve: Influence on Fibroblasts, Extracellular Matrix, and Potential Stenosis Sven Lehmann, MD, Thomas Walther, MD,

In vitro differences between smooth muscle cells derived from varicose veins and normal veins Ying Xiao, PhD, Zhibin Huang, MD, Henghui Yin, PhD, Ying.

Fitsum Lakew, MD, Paul P. Urbanski, MD, PhD

Mitral Valve Necrosis After Cardiac Surgery Using Gelatin-Resorcinol-Formaldehyde Glue Yuki Tanaka, MD, Tadashi Kitamura, MD, PhD, Yurie Miyata, MD,

Clinical pulmonary autograft valves: Pathologic evidence of adaptive remodeling in the aortic site Elena Rabkin-Aikawa, MD, PhD, Masanori Aikawa, MD,

Identification of opticin, a member of the small leucine-rich repeat proteoglycan family, in human articular tissues: a novel target for MMP-13 in osteoarthritis

Natural history of thoracic aortic aneurysms: indications for surgery, and surgical versus nonsurgical risks John A Elefteriades, MD The Annals of Thoracic.

Müllerian-inhibiting substance inhibits cytochrome P450 aromatase activity in human granulosa lutein cell culture Michael P. Grossman, M.D., Steven T.

Hans-Joachim Schäfers, MD, PhD, Diana Aicher, MD, Frank Langer, MD

Transapical Aortic Valve Implantation in High-Risk Patients With Severe Aortic Valve Stenosis Augusto D'Onofrio, MD, Melissa Fusari, MD, Nicola Abbiate,

IgG Autoantibodies from Bullous Pemphigoid Patients Recognize Multiple Antigenic Reactive Sites Located Predominantly Within the B and C Subdomains of.

Aortic valve replacement: comparison of late survival between autografts and homografts Christopher J Knott-Craig, MD, Ronald C Elkins, MD, KathyLee.

Qing-yu Wu, MD The Annals of Thoracic Surgery

Incidence and predictors of supraventricular dysrhythmias after pulmonary resection Jack J Curtis, MD, Brent M Parker, MD, Charlotte A McKenney, RN, Colette.

John E Langenfeld, MD The Annals of Thoracic Surgery

Zohair Al-Halees, FRCSC, Fadel Al-Fadley, FRCPC

“Transitions” for Cardiothoracic Surgeons Now Listed on the Web:

Six-year monitoring of the donor-specific immune response to cryopreserved aortic allograft valves: Implications with valve dysfunction Giulio Pompilio,

Surgical management of mitral regurgitation associated with marfan’s syndrome Jean-François G Fuzellier, MD, Sylvain M Chauvaud, MD, Paul Fornes, MD,

Pulmonary artery banding before Norwood procedure

Development of abnormal tissue architecture in transplanted neonatal rat myocytes Peter Whittaker, PhD, Jochen Müller-Ehmsen, MD, Joan S Dow, BS, Larry.

Mark I. Block, MD The Annals of Thoracic Surgery

Double criss-cross sternal wiring and chest wound infections

KDM4A levels affect the distribution of translation initiation factors

Presentation transcript:

Cell composition of the human pulmonary valve: a comparative study with the aortic valve–the VESALIO∗ project∗ Foscarina Della Rocca, MD, Saverio Sartore, PhD, Diego Guidolin, PhD, Barbara Bertiplaglia, BSc, Gino Gerosa, MD, Dino Casarotto, MD, Paolo Pauletto, MD The Annals of Thoracic Surgery Volume 70, Issue 5, Pages 1594-1600 (November 2000) DOI: 10.1016/S0003-4975(00)01979-2

Fig 1 Immunocytochemistry on cryosections from pulmonary valve leaflets (A, C, E, G) and aortic valve leaflets (B, D, F, H) reacted with anti-SM α-actin (A, B), anti-SM22 (C, D), anticalponin (E, F), and anti-SM(-MyHC) myosin (G, H), monoclonal antibodies. Note the continuous subendocardial layer of positive cells in A (green arrows) and clusters of positive cells in C, E, G, and H (red arrows) in the outermost part of the fibrosa. In aortic valve leaflets, the layer of positive subendocardial cells is generally thicker than that of pulmonary valve leaflets (× 400). The Annals of Thoracic Surgery 2000 70, 1594-1600DOI: (10.1016/S0003-4975(00)01979-2)

Fig 2 Bar graph showing the distribution of positive cells (number of cells/μm2) with (A) antivimentin, (B) NM-F6 anti-MyHC-Apla1, and (C) NM-G2 anti-MyHC-Apla2 in fibrosa, spongiosa, and ventricularis from aortic valve leaflets (white bars) and pulmonary valve leaflets (black bars). Mean ± SD is reported. The Annals of Thoracic Surgery 2000 70, 1594-1600DOI: (10.1016/S0003-4975(00)01979-2)

Fig 3 Western blotting analysis of crude extracts from (1) aortic valve leaflets and (2) pulmonary valve leaflets electrophoresed in (A) 12.5% or (B) 5% SDS-gels, reacted with antivimentin, anti-SM22, and anti-SM (α)-actin is shown in panel A. In panel B, the pattern obtained with anti-SM(-MyHC), anti-MyHC-Apla1, and anti-MyHC-Apla2 antibodies is shown. The band reactive with the anti-NM myosin antibodies is about 200-kDa apparent mass. Note that SM22 and SM-MyHC antigens are undetectable under the conditions used in this experiment; a, b, and c indicated the Western blots. The Annals of Thoracic Surgery 2000 70, 1594-1600DOI: (10.1016/S0003-4975(00)01979-2)

Fig 4 Schematic representation of the various cell populations present in the fibrosa, spongiosa, and ventricularis of aortic valve leaflets (AVL) and pulmonary valve leaflets (PVL) as determined by immunocytochemical experiments. Fetal-type SM cells and myofibroblasts are segregated in the outermost layer of fibrosa, whereas different fibroblast subtypes are expressed in the ventricularis of AVL versus PVL and in the spongiosa of both valves as well. The slight difference existing in vimentin distribution between AVL and PVL at the level of fibrosa is not reported in the figure. The up arrows and down arrows indicate a high or low number of vimentin-positive cells, respectively. The Annals of Thoracic Surgery 2000 70, 1594-1600DOI: (10.1016/S0003-4975(00)01979-2)