Ex vivo expanded unselected peripheral blood: progenitor cells reduce posttransplantation neutropenia, thrombocytopenia, and anemia in patients with breast.

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Ex vivo expanded unselected peripheral blood: progenitor cells reduce posttransplantation neutropenia, thrombocytopenia, and anemia in patients with breast cancer by Ronald L. Paquette, Sanaa T. Dergham, Ellen Karpf, He-Jing Wang, Dennis J. Slamon, Larry Souza, and John A. Glaspy Blood Volume 96(7):2385-2390 October 1, 2000 ©2000 by American Society of Hematology

Effect of cell culture density on stem cell expansion and myeloid cell yield.Unselected PBPC were seeded into cultures at 1 of 3 different cell densities. Effect of cell culture density on stem cell expansion and myeloid cell yield.Unselected PBPC were seeded into cultures at 1 of 3 different cell densities. (A) Fold expansion was calculated by dividing the number of viable myeloid cells obtained after 9 days of culture by the number of CD34+ cells at culture initiation. (B) Myeloid cell yield was determined by dividing the number of viable myeloid cells harvested on day 9 by the total number of PBPC seeded into the cultures. Results are reported as the mean ± SD. Statistics were calculated using analysis of variance. Ronald L. Paquette et al. Blood 2000;96:2385-2390 ©2000 by American Society of Hematology

Immunophenotype of ex vivo expanded cells Immunophenotype of ex vivo expanded cells.PBPC were subjected to immunofluorescent staining on days 0 and 9 of culture using FITC-conjugated anti-CD15 and phycoerythrin-conjugated anti-CD11b, or isotype controls, then analyzed by flow cytometry. Immunophenotype of ex vivo expanded cells.PBPC were subjected to immunofluorescent staining on days 0 and 9 of culture using FITC-conjugated anti-CD15 and phycoerythrin-conjugated anti-CD11b, or isotype controls, then analyzed by flow cytometry. Ronald L. Paquette et al. Blood 2000;96:2385-2390 ©2000 by American Society of Hematology

Effect of expanded cell dose on the time to neutrophil recovery Effect of expanded cell dose on the time to neutrophil recovery.The number of ex vivo expanded cells administered was correlated with the time to neutrophil recovery after cell infusion. Effect of expanded cell dose on the time to neutrophil recovery.The number of ex vivo expanded cells administered was correlated with the time to neutrophil recovery after cell infusion. A strong linear relationship between these variables is suggested by the correlation coefficient, r = −0.80. Ronald L. Paquette et al. Blood 2000;96:2385-2390 ©2000 by American Society of Hematology

Effect of expanded cell dose on the duration of neutropenia Effect of expanded cell dose on the duration of neutropenia.The number of ex vivo expanded cells infused was correlated with the duration of neutropenia (r = −0.74). Effect of expanded cell dose on the duration of neutropenia.The number of ex vivo expanded cells infused was correlated with the duration of neutropenia (r = −0.74). Ronald L. Paquette et al. Blood 2000;96:2385-2390 ©2000 by American Society of Hematology

Effect of CD34+ cell number on the time to neutrophil recovery Effect of CD34+ cell number on the time to neutrophil recovery.The day of neutrophil recovery was correlated with the CD34+ cell content of the PBPC seeded into culture (r = −0.67). Effect of CD34+ cell number on the time to neutrophil recovery.The day of neutrophil recovery was correlated with the CD34+ cell content of the PBPC seeded into culture (r = −0.67). Ronald L. Paquette et al. Blood 2000;96:2385-2390 ©2000 by American Society of Hematology

Effect of expanded cell dose on the number of red cell transfusions Effect of expanded cell dose on the number of red cell transfusions.The number of ex vivo expanded cells given was correlated with the number of red blood cell units transfused (r = −0.61). Effect of expanded cell dose on the number of red cell transfusions.The number of ex vivo expanded cells given was correlated with the number of red blood cell units transfused (r = −0.61). Ronald L. Paquette et al. Blood 2000;96:2385-2390 ©2000 by American Society of Hematology

Effect of expanded cell dose on the time to platelet recovery Effect of expanded cell dose on the time to platelet recovery.The number of ex vivo expanded cells administered was correlated with the time to platelet recovery after cell infusion (r = −0.49). Effect of expanded cell dose on the time to platelet recovery.The number of ex vivo expanded cells administered was correlated with the time to platelet recovery after cell infusion (r = −0.49). Ronald L. Paquette et al. Blood 2000;96:2385-2390 ©2000 by American Society of Hematology

Effect of expanded cell dose on the duration of hospitalization Effect of expanded cell dose on the duration of hospitalization.The number of ex vivo expanded cells administered was associated with the length of hospital stay (r = −0.62). Effect of expanded cell dose on the duration of hospitalization.The number of ex vivo expanded cells administered was associated with the length of hospital stay (r = −0.62). Ronald L. Paquette et al. Blood 2000;96:2385-2390 ©2000 by American Society of Hematology