Chapter 12 Structure of Nucleic Acids.

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Presentation transcript:

Chapter 12 Structure of Nucleic Acids

12.1 The Primary Structure of Nucleic Acids

The chain termination or dideoxy method of DNA sequencing

Automated DNA Sequencing

12.2 Secondary Structure of DNA DNA double helix right-handed 10bp per turn 0.34nm d = 2nm

Base stacking force

antiparallel

12.3 Denaturation and Renaturation of DNA 12.3.1 DNA Denaturation

12.3.2 DNA Renaturation

12.3.3 Nucleic Acid Hybridization 12.3.4 Buoyant Density of DNA

Density gradient centrifugation

12.4 Supercoil and Cruciform : Tertiary structure in DNA

Relaxed DNA Superhelix DNA Unwound DNA

cruciform Palindromic sequence

12.5 Secondary and Tertiary Structure of RNA Unusual bases of RNA

12.5.1 Messenger RNA ( mRNA ) 5`-cap 3`-poly(A) 7-methyl-G-cap

12.5.2 Transfer RNA ( tRNA ) Cloverleaf secondary structure of tRNA

Tertiary interactions in yeast phenylalanine tRNA

L-shaped tertiary form

12.5.3 Ribosomal RNA ( rRNA )

The proposed secondary structure for E.coli 16S rRNA

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