HDAC3, but not HDAC2 is in complex with tau protein.

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HDAC3, but not HDAC2 is in complex with tau protein. HDAC3, but not HDAC2 is in complex with tau protein. (A) Representative immunoblots showing the interaction between HDAC3 and tau in HEK/APPsw cells following co-IP with anti-HDAC3 antibody. HEK/APPsw protein lysate was prepared and co-IP analysis was carried out followed by immunoblotting with antibodies against HDAC2, HDAC3, tau, and GAPDH. No interaction was detected between HDAC3 and GAPDH (negative control). IgG immunoprecipitates were used as negative controls for protein binding. HEK/APPsw cell lysates (input) served as positive control. (B) Representative immunoblots showing that 48 h of 10 μM of RGFP-966 treatment increased binding of HDAC3 to tau in HEK/APPsw cells. Presented bands illustrate variability of independent experiments (IP lanes). HDAC2 and GAPDH were used as a negative control (no complex detected). n = 3. Karolina J. Janczura et al. PNAS 2018;115:47:E11148-E11157 ©2018 by National Academy of Sciences