Gene transfer to vein graft wall by HVJ–liposome method: time course and localization of gene expression Hong-zhi Bai, MD, Yoshiki Sawa, MD, Wei-da Zhang,

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Presentation transcript:

Gene transfer to vein graft wall by HVJ–liposome method: time course and localization of gene expression Hong-zhi Bai, MD, Yoshiki Sawa, MD, Wei-da Zhang, MD, Tomoyuki Yamakawa, MD, Ryuichi Morishita, MD, Yasufumi Kaneda, MD, Hikaru Matsuda, MD The Annals of Thoracic Surgery Volume 66, Issue 3, Pages 814-819 (September 1998) DOI: 10.1016/S0003-4975(98)00594-3

Fig 1 (A) Preparation method for hemagglutinating virus of Japan (HVJ)–liposome complex and (B) procedures to transfect vein graft ex vivo. chol/TF = cholesterol/; DNA = deoxyribonucleic acid; HMg-1 = high-mobility group 1; ODN = oligodeoxynucleotide; PL = phosphatidylcholine; PS = phosphatidylserine; TS = transfection.) The Annals of Thoracic Surgery 1998 66, 814-819DOI: (10.1016/S0003-4975(98)00594-3)

Fig 2 Gross examination of en bloc segments of vein grafts exposed to hemagglutinating virus of Japan-liposomes containing either (A) β-galactosidase plasmid DNA or (B) no genes. (X-gal; ×8 before 38% reduction.) The Annals of Thoracic Surgery 1998 66, 814-819DOI: (10.1016/S0003-4975(98)00594-3)

Fig 3 Microscopic views of sections from vein grafts exposed to hemagglutinating virus of Japan-liposomes containing either (A, C) β-galactosidase (β-gal) plasmid DNA or (B, D) no genes. All sections were stained with X-gal to detect the β-gal expression before sectioning, and counterstaining was done with hematoxylin in C and D. The arrows indicate borders between neointima and media. (×200 before 19% reduction.) The Annals of Thoracic Surgery 1998 66, 814-819DOI: (10.1016/S0003-4975(98)00594-3)

Fig 4 Microscopic views of sections from vein grafts exposed to fluorescein isothiocyanate–labeled phosphorothioate oligodeoxynucleotides either (A, C, E) with hemagglutinating virus of Japan-liposomes or (B, D, F) without them. The grafts were harvested on day 4 in A, B, C, and D and on day 14 in E and F. All sections were incubated in erichrome black T solution to diminish the autofluorescence from elastin and were observed with a fluorescent microscope equipped with differential interference contrast apparatus. The arrows indicate the borders between neointima and media. (A, B: ×200 before 14% reduction; C, D, E, F: ×400 before 14% reduction.) The Annals of Thoracic Surgery 1998 66, 814-819DOI: (10.1016/S0003-4975(98)00594-3)