Microscope Calculations

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Presentation transcript:

Microscope Calculations

Total Magnification To calculate the total magnification of an image that you are viewing through the microscope take the power of the objective (4X, 10X, 40x) and multiply by the power of the eyepiece, usually 10X.

Estimating the Size of an Object Size of object = diameter of field of view (µm) number of times the object fits across the field of view **Teacher will give you diameter!

This triangicium is being viewed under medium power. How many times does the triangicium fit across the field of view? low power FOV = 4200 μm

This diamondogram is being viewed under high power. How many times does the diamondogram cross the field of view? low power FOV = 3.5mm

How to Make a Wet Mount Slide Gather a thin slice/piece of specimen. If your specimen is too thick, then the coverslip will wobble on top of the sample like a see-saw Place ONE drop of water directly over the specimen. If you put too much water over the specimen, then the coverslip will float on top of the water. Place the coverslip at a 45 degree angle (approximately), with one edge touching the water drop, and let go.

How to Stain a Slide Place one drop of Methylene Blue stain on one edge of the coverslip, and the flat edge of a piece of paper towel on the other edge of the coverslip. The paper towel will draw the water out from under the coverslip. As soon as the stain has covered the area containing the specimen you are finished. The stain does not need to be under the entire coverslip. If the stain does not cover the area needed, get a new piece of paper towel and add more stain until it does.

Biological Drawings

Drawings, labels, name and other information must be done in pencil. Use white 8 ½ by 11 paper. The drawing should be as large as possible and kept to the center of the page. The title of the drawing is simply the name of the object you are looking at. All labels are printed and lined up on the right hand side. Draw just what you see. Do not add structures because you think they should be there. Do not shade or sketch.

What you are looking for in Onion cells… Low/ Med power High power What you are looking to label

What you are looking for in Cheek cells… Low power Med power High power What you are looking to label