Volume 39, Issue 5, Pages (November 2003)

Slides:



Advertisements
Similar presentations
Volume 59, Issue 5, Pages (May 2001)
Advertisements

Bcl-2 overexpression prevents apoptosis-induced Madin-Darby canine kidney simple epithelial cyst formation1  Hsi-Hui Lin, Tzi-Peng Yang, Si-Tse Jiang,
Volume 125, Issue 1, Pages (July 2003)
L. Nikitina, F. Wenger, M. Baumann, D. Surbek, M. Körner, C. Albrecht 
Gene-Corrected Fibroblast Therapy for Recessive Dystrophic Epidermolysis Bullosa using a Self-Inactivating COL7A1 Retroviral Vector  Joanna Jacków, Matthias.
Identification of the Major Intestinal Fatty Acid Transport Protein
Expression of Human Macrophage Metalloelastase (MMP-12) by Tumor Cells in Skin Cancer  Erja Kerkelä, Risto Ala-aho, Leila Jeskanen, Oona Rechardt, Reidar.
Volume 125, Issue 1, Pages (July 2003)
Elevation of hemopexin-like fragment of matrix metalloproteinase-2 tissue levels inhibits ischemic wound healing and angiogenesis  April E. Nedeau, MD,
Volume 134, Issue 3, Pages (March 2008)
Volume 23, Issue 4, Pages (April 2018)
Macrophage-Derived Metalloelastase Is Responsible for the Generation of Angiostatin in Lewis Lung Carcinoma  Zhongyun Dong, Rakesh Kumar, Xiulan Yang,
Impaired glycolytic metabolism causes chondrocyte hypertrophy-like changes via promotion of phospho-Smad1/5/8 translocation into nucleus  T. Nishida,
Alterations in vesicle transport and cell polarity in rat hepatocytes subjected to mechanical or chemical cholestasis  Natalie J. Török, Elizabeth M.
Proliferation, Cell Cycle Exit, and Onset of Terminal Differentiation in Cultured Keratinocytes: Pre-Programmed Pathways in Control of C-Myc and Notch1.
Unprocessed Interleukin-36α Regulates Psoriasis-Like Skin Inflammation in Cooperation With Interleukin-1  Katelynn A. Milora, Hangfei Fu, Ornella Dubaz,
Volume 128, Issue 5, Pages (May 2005)
Volume 80, Issue 12, Pages (December 2011)
Richard T. Ethridge, Mark R. Hellmich, Raymond N. DuBois, B.Mark Evers 
Fetal liver stroma consists of cells in epithelial-to-mesenchymal transition by Jalila Chagraoui, Adeline Lepage-Noll, Aurora Anjo, Georges Uzan, and Pierre.
Volume 132, Issue 2, Pages (February 2007)
Volume 117, Issue 5, Pages (November 1999)
Fibroblast growth factor-2 induced chondrocyte cluster formation in experimentally wounded articular cartilage is blocked by soluble Jagged-1  I.M. Khan,
Regulation of Hair Shedding by the Type 3 IP3 Receptor
Volume 124, Issue 1, Pages (January 2003)
Volume 115, Issue 2, Pages (August 1998)
Volume 60, Issue 2, Pages (August 2001)
Role of Monocyte Chemoattractant Protein-1 and its Receptor,CCR-2, in the Pathogenesis of Bleomycin-Induced Scleroderma  Toshiyuki Yamamoto, Kiyoshi Nishioka 
Volume 128, Issue 5, Pages (May 2005)
Volume 61, Issue 5, Pages (May 2002)
Volume 128, Issue 7, Pages (June 2005)
Volume 114, Issue 3, Pages (March 1998)
Volume 115, Issue 1, Pages (July 1998)
Bcl-2 overexpression prevents apoptosis-induced Madin-Darby canine kidney simple epithelial cyst formation1  Hsi-Hui Lin, Tzi-Peng Yang, Si-Tse Jiang,
Spleen Tyrosine Kinase Mediates EGFR Signaling to Regulate Keratinocyte Terminal Differentiation  Nan-Lin Wu, Duen-Yi Huang, Li-Fang Wang, Reiji Kannagi,
Collagen XVII Shedding Suppresses Re-Epithelialization by Directing Keratinocyte Migration and Dampening mTOR Signaling  Joanna Jacków, Stefanie Löffek,
Volume 59, Issue 5, Pages (May 2001)
Enhancing 1α-Hydroxylase Activity with the 25-Hydroxyvitamin D-1α-Hydroxylase Gene in Cultured Human Keratinocytes and Mouse Skin  Tai C. Chen, Xue Hong.
Endogenous hepatocyte growth factor ameliorates chronic renal injury by activating matrix degradation pathways  Youhua Liu, Krupa Rajur, Evelyn Tolbert,
Role of Connective Tissue Growth Factor in Oval Cell Response During Liver Regeneration After 2-AAF/PHx in Rats  Liya Pi, Seh-Hoon Oh, Thomas Shupe, Bryon.
Volume 39, Issue 5, Pages (November 2003)
Volume 39, Issue 2, Pages (August 2003)
A new mouse model of immune-mediated podocyte injury
Volume 135, Issue 2, Pages (August 2008)
Volume 134, Issue 3, Pages (March 2008)
Mohammad Rashel, Ninche Alston, Soosan Ghazizadeh 
Volume 54, Issue 4, Pages (October 1998)
Volume 41, Issue 2, Pages (August 2004)
Volume 129, Issue 5, Pages (November 2005)
A novel model to study renal myofibroblast formation in vitro
Bcl-2 overexpression prevents apoptosis-induced Madin-Darby canine kidney simple epithelial cyst formation1  Hsi-Hui Lin, Tzi-Peng Yang, Si-Tse Jiang,
Volume 75, Issue 5, Pages (March 2009)
Superoxide-induced apoptosis of activated rat hepatic stellate cells
Collagen VII Half-Life at the Dermal-Epidermal Junction Zone: Implications for Mechanisms and Therapy of Genodermatoses  Tobias Kühl, Markus Mezger, Ingrid.
Akito Maeshima, Yoshihisa Nojima, Itaru Kojima  Kidney International 
The Function of Nitric Oxide in Wound Repair: Inhibition of Inducible Nitric Oxide- Synthase Severely Impairs Wound Reepithelialization  Birgit Stallmeyer,
Aortic wall cell proliferation via basic fibroblast growth factor gene transfer limits progression of experimental abdominal aortic aneurysm  Katsuyuki.
Epithelial Cells in the Hair Follicle Bulge do not Contribute to Epidermal Regeneration after Glucocorticoid-Induced Cutaneous Atrophy  Dmitry V. Chebotaev,
Volume 44, Issue 2, Pages (February 2006)
Stacy Mazzalupo, Matthew J. Wawersik, Pierre A. Coulombe 
Volume 39, Issue 3, Pages (September 2003)
IFN-τ inhibits IgE production in a murine model of allergy and in an IgE-producing human myeloma cell line  Mustafa G. Mujtaba, PhDa, Lorelie Villarete,
Nur Hayati Jaafar Marican, Sara B. Cruz-Migoni, Anne-Gaëlle Borycki 
Volume 9, Issue 8, Pages (April 1999)
Volume 39, Issue 1, Pages (July 2003)
Normal Human Merkel Cells are Present in Epidermal Cell Populations Isolated and Cultured from Glabrous and Hairy Skin Sites  Julie Fradette, Danielle.
Gene-Corrected Fibroblast Therapy for Recessive Dystrophic Epidermolysis Bullosa using a Self-Inactivating COL7A1 Retroviral Vector  Joanna Jacków, Matthias.
Identification of the Major Intestinal Fatty Acid Transport Protein
Volume 44, Issue 6, Pages (June 2006)
Matrix Metalloproteinase Inhibitor BB-3103 Unlike the Serine Proteinase Inhibitor Aprotinin Abrogates Epidermal Healing of Human Skin Wounds Ex Vivo1 
Presentation transcript:

Volume 39, Issue 5, Pages 716-723 (November 2003) Rapid formation of hepatic organoid in collagen sponge by rat small hepatocytes and hepatic nonparenchymal cells  Keisuke Harada, Toshihiro Mitaka, Shigeki Miyamoto, Shinichi Sugimoto, Shinichiro Ikeda, Hiroshi Takeda, Yohichi Mochizuki, Koichi Hirata  Journal of Hepatology  Volume 39, Issue 5, Pages 716-723 (November 2003) DOI: 10.1016/S0168-8278(03)00412-4

Fig. 1 Experimental protocol: SH colonies were isolated from the culture dishes at 12–14 days after plating and replated on collagen sponges (CS) or collagen-coated dishes (C). Black arrowheads indicate the days for collecting the medium, whereas white arrowheads indicate the days of 2 mM NH4Cl treatment. Journal of Hepatology 2003 39, 716-723DOI: (10.1016/S0168-8278(03)00412-4)

Fig. 2 Phase-contrast photographs of SH colonies replated on a new collagen-coated dish. The cells within the same field marked by a needle are shown. (A) Day 3. SH colonies attached on the dishes. NPCs are shown by arrows. (B) Day 6. The proliferation of SHs can be clearly observed and NPCs also proliferate around the colonies. (C) Day 9. (D) Day 13. The proliferation of NPCs is suppressed, whereas SHs continue growing. (E) Day 16. (F) Day 28. All photographs show the same magnification. Scale bar, 50 μm. Journal of Hepatology 2003 39, 716-723DOI: (10.1016/S0168-8278(03)00412-4)

Fig. 3 Photographs of replated SH colonies on a collagen sponge. (A) Day 7 after replating. Hematoxylin-eosin staining was carried out. SH colonies attached on the collagen fibers. (B) Day 56. The dark violet shows the cell aggregates (arrowheads). They seem to be hepatic organoids. Scale bar, 150 μm. (C) DNA synthesis of the cells replated on a collagen sponge is shown by the immunocytochemistry for BrdU. The cells were treated with 40 μM BrdU at day 7 and, 24 h later, fixed with cold absolute ethanol. The cells with brown nuclei incorporated BrdU into their DNA. Scale bar, 50 μm. Journal of Hepatology 2003 39, 716-723DOI: (10.1016/S0168-8278(03)00412-4)

Fig. 4 Perpendicular sections of the collagen sponge are shown. Hematoxylin-eosin staining (A–C) and immunocytochemistry for albumin (D), CK8 (E), CK19 (F), desmin (G), ED1/2 (H), and SE-1 (I) were carried out. (A) Day 7. (B) Day 21. (C) Day 56. Scale bars, 30 μm. Albumin and CK8 were used as markers for hepatocytes, CK19 for bile duct cells, desmin for stellate cells, ED1/2 for Kupffer cells, and SE1 for sinusoidal endothelial cells. The cells positive for the antibody are stained green. E-cadherin or β-catenin (red) was used for the identification of epithelial cells and the cells positive for the antibody are stained red. DAPI (blue) was used for the identification of nuclei. The images of these triple stainings were taken by fluorescent microscopy and analyzed with the CELLScan system. (D–I) Day 28. Scale bar, 20 μm. Journal of Hepatology 2003 39, 716-723DOI: (10.1016/S0168-8278(03)00412-4)

Fig. 5 Transmission electron micrographs of a perpendicular section of cells in a collagen sponge at day 21. The cells have many mitochondria, rER, peroxisomes, and glycogen granules in their cytoplasm. (A) Arrows show bile canaliculi between cells. Scale bar, 2 μm. (B) Large peroxisomes with a crystalline nucleoid (arrows) are observed in the cytoplasm. Scale bar, 500 nm. (C) Arrows show gap junctions and arrowheads show desmosomes between hepatocytes. Scale bar, 200 nm. Journal of Hepatology 2003 39, 716-723DOI: (10.1016/S0168-8278(03)00412-4)

Fig. 6 (A) Albumin secretion into the culture medium of the cells replated on collagen sponges; 1.25×103 (circle), 2.5×103 (square), 5×103 (diamond), and 10×103 (triangle) colonies were replated on collagen sponges. The details are described in Materials and methods. Three independent experiments were performed. The points show the average of three dishes and the bars S.D. (B) Albumin secretion of the cells cultured in collagen-coated dishes (white) and in collagen sponges (black); 2.5×103 (squares) and 1.25×103 (circles) colonies are shown. Three independent experiments were performed. The points show the average of three dishes and the bars S.D. Journal of Hepatology 2003 39, 716-723DOI: (10.1016/S0168-8278(03)00412-4)

Fig. 7 Western blot analysis for transferrin, haptoglobin, and fibrinogen of the medium (A) and CPS and CYP 1A1 of the cells cultured on the collagen sponge (B); 2.5×103 colonies were replated on collagen sponges (CS) and on collagen-coated dishes (C). Samples (medium, 1 μl/lane; cells, 10 μg/lane) were separated by 10% SDS-PAGE. NS, normal rat serum or plasma (×1000 diluted with PBS); P, primary MHs at 3 h after plating. (C) Northern blot analysis for albumin and transferrin mRNAs of the cells cultured on the collagen sponge. For each lane, 20 μg of total RNA was applied and hybridized with each cDNA probe after blotting. Details are described in Materials and methods. Journal of Hepatology 2003 39, 716-723DOI: (10.1016/S0168-8278(03)00412-4)

Fig. 8 Urea synthesis of the cells cultured in collagen sponges (squares) and collagen coated dishes (circles). The medium was replaced with that supplemented with 2 mM NH4Cl at days 7 and 14 after replating. Twenty-four hours later, the medium was collected. The methods are described in Materials and methods. Three independent experiments were performed. The points show the average of three dishes and the bars S.D. Journal of Hepatology 2003 39, 716-723DOI: (10.1016/S0168-8278(03)00412-4)

Fig. 9 Corresponding phase-contrast (A and C) and fluorescent images (B and D). Uptake and metabolization of FD by cells in the sponges at day 56. Secretion of fluorescence into bile canaliculi is clearly shown. BC are organized between the cells cultured in collagen sponges (arrows). All photographs show the same magnification. Scale bar, 30 μm. Journal of Hepatology 2003 39, 716-723DOI: (10.1016/S0168-8278(03)00412-4)