Differential block of high voltage-activated CaV channel currents in DRG neurons by WT and mutant RGK proteins. Differential block of high voltage-activated.

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Differential block of high voltage-activated CaV channel currents in DRG neurons by WT and mutant RGK proteins. Differential block of high voltage-activated CaV channel currents in DRG neurons by WT and mutant RGK proteins. (A) Representative images of cultured DRG neurons expressing GFP, CFP-Rad, or CFP-Rad[R208A/L235A]. (B) Exemplar IBa waveforms elicited by voltage-ramp protocols in DRG neurons expressing GFP (Left) or CFP-Rad (Right). (C) Exemplar family of HVA IBa from DRG neurons expressing GFP (Left), CFP-Rad (Middle), or CFP-Rad[208A/L235A] (Right). Currents were elicited from a holding potential of −50 mV and in the presence of 1 μM mibefradil to eliminate LVA T-type currents. (D) Bar chart showing the relative impact of distinct WT and β-binding–deficient mutant RGKs on HVA CaV1/CaV2 channel currents in cultured DRG neurons. Data are means ± SEM. *P < 0.05, one-way ANOVA and post hoc Bonferroni test. Akil A. Puckerin et al. PNAS 2018;115:47:12051-12056 ©2018 by National Academy of Sciences