Ogami_Fig. S1 CPEB pHA-CMV Time (h) oligo(dT)/ RNase H

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Presentation transcript:

Ogami_Fig. S1 CPEB pHA-CMV5- 1.5 3 Time (h) oligo(dT)/ RNase H 1.5 3 Time (h) oligo(dT)/ RNase H GST-CAT mRNA BGG c-myc 3’UTR mRNA 1 2 3 4 5 6 7 8 9 10

A B C Ogami_Fig. S2 MS2-HA -CPEB ( ) MS2-HA ( ) HA-CPEB ( ) (kb) 2 4 6 ( ) HA-CPEB ( ) (kb) 2 4 6 8 2 4 6 8 2 4 6 8 Time (h) 1.5 EGFP mRNA 1.0 0.8 BGG(1-39) MS2bs mRNA 0.6 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 B C 100 MS2-HA-CPEB MS2-HA HA-CPEB (kDa) RNA level (%) 89 MS2-HA-CPEB 10 63 HA-CPEB MS2-HA (WB: anti-HA) MS2-HA-CPEB GAPDH HA-CPEB 35 (WB: anti-GAPDH) 1 2 4 6 8 Time (h)

Ogami_Fig. S3 pCMV-5XMyc-PARN CPEB CPEB3 CPEB CPEB3 pCMV-5XFlag- anti-Flag anti-Flag anti-Flag (kDa) IP 93 5XMyc-PARN (WB: anti-Myc) 5XFlag-CPEB3 93 5XFlag-CPEB 62 (WB: anti-Flag) 1 2 3 4 5 6

Ogami_Fig. S4 A B mature (a-c) pre (b-c) Maker RT