iGEM Meeting #7 08/06/08 Presentation by Robert Ovadia

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Presentation transcript:

iGEM Meeting #7 08/06/08 Presentation by Robert Ovadia Lactose Intolerance iGEM Meeting #7 08/06/08 Presentation by Robert Ovadia

iGEM Meeting #7 08/06/08 Presentation by Robert Ovadia System Design The idea behind using RBS’s is we want to use the weakest RBS for lacY so we can pump the strongest promoter. We want to express LacZ as much in order to do this. *ADD ORI’s* iGEM Meeting #7 08/06/08 Presentation by Robert Ovadia

iGEM Meeting #7 08/06/08 Presentation by Robert Ovadia System Design By next week, you guys should be telling me how the plasmids look like. I will test you! *ORI’s* iGEM Meeting #7 08/06/08 Presentation by Robert Ovadia

Two Weeks Left The Final Push: What We Want 33 LacY 34 LacZ SDM +2H We have: 33 LacY 34 LacZ SDM +H SDM +2H?? iGEM Meeting #7 08/06/08 Presentation by Robert Ovadia

Two Weeks Left The Final Push: What We Want Lysis Cassette A81 / B4 In pSB2K3 We have: Lysis Cassette In pSB2K3 iGEM Meeting #7 08/06/08 Presentation by Robert Ovadia