MOSPD2 recruitment to interorganelle contact sites depends on the interaction between the FFAT motif and the MSP domain MOSPD2 recruitment to interorganelle.

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MOSPD2 recruitment to interorganelle contact sites depends on the interaction between the FFAT motif and the MSP domain MOSPD2 recruitment to interorganelle contact sites depends on the interaction between the FFAT motif and the MSP domain A–D GFP‐MOSPD2 RD/LD (green)‐expressing cells were transfected with Flag‐STARD3 (A), Flag‐ORP1L (B), Flag‐STARD11 (C), or HA‐PTPIP51 (D), and labeled using anti‐Flag (A–C; magenta), anti‐HA (D: magenta), and anti‐Lamp1 (A and B; red), GM130 (C; red), or OPA‐1 (D; red), as markers of late endosomes/lysosomes, Golgi, and mitochondria, respectively. The subpanels on the right are higher magnification (3.5×) images of the area outlined in white. The Overlay panel shows merged green and magenta images. Scale bars: 10 μm.EPearson correlation coefficients between MOSPD2 (WT or RD/LD mutant) and STARD3 (WT or FA/YA), ORP1L (WT or FA/YA), STARD11 (WT or D324A), and PTPIP51 (WT or ΔFFAT 1–2) staining are shown. Each dot represents a single cell from three independent experiments (number of cells: MOSPD2–STARD3: 12; MOSPD2–STARD3 FA/YA: 16; MOSPD2 RD/LD–STARD3: 12; MOSPD2–ORP1L: 19; MOSPD2–ORP1L FA/YA: 23; MOSPD2 RD/LD–ORP1L: 17; MOSPD2–STARD11: 21; MOSPD2–STARD11 D324A: 19; MOSPD2 RD/LD–STARD11: 18; MOSPD2–PTPIP51: 15; MOSPD2–PTPIP51 ΔFFAT 1–2: 10; MOSPD2 RD/LD–PTPIP51: 10). Means and error bars (SD) are shown. Kruskal–Wallis with Dunn's multiple comparison test (*P‐values < 0.05; **P < 0.01; ***P < 0.001). Thomas Di Mattia et al. EMBO Rep. 2018;19:e45453 © as stated in the article, figure or figure legend