Tumour‐derived miRNAs are delivered to endothelial cells via microvesicles. Tumour‐derived miRNAs are delivered to endothelial cells via microvesicles.

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Tumour‐derived miRNAs are delivered to endothelial cells via microvesicles. Tumour‐derived miRNAs are delivered to endothelial cells via microvesicles. (A) Quantitative PCR of miRNAs in HUVECs stimulated with VEGF (50 ng/ml), or co‐cultured with parental (C2P) or VEGF null (G5) fibrosarcoma cells. *P<0.05, Student's t‐test. (B) Quantitative PCR of miR‐9 in HUVECs co‐cultured with different fractions of SK23 conditioned medium after anion exchange chromatography. The experiment was repeated twice. (C) Quantitative PCR of miR‐9 in HUVECs co‐cultured with different fractions of SK23 conditioned medium after heparin‐sepharose chromatography. The experiment was repeated twice. (D) Conditioned medium was collected from each cancer cell line and miRNAs concentration was measured by quantitative RT–PCR against spike‐in synthetic oligonucleotides. (E) Quantitative PCR of miRNAs in HUVECs treated with SK23 conditioned medium, SK23 microvesicles or 4T1 microvesicles. Three biological replicates were used for each condition. *P<0.05, Student's t‐test. (F) SK23 microvesicles were labelled with PKH67 (green), concentrated with Amicon filters (30KDa) and incubated with HUVECs for 24 hours (left panel). SK23 cells were labelled with DiI (red) and co‐cultured with HUVECs (middle panel). Purified microvesicles from DiI‐labelled SK23 were concentrated with Amicon filters (30KDa) and added to HUVECs (right panel). The experiment was repeated twice. Guanglei Zhuang et al. EMBO J. 2012;31:3513-3523 © as stated in the article, figure or figure legend