FcγRIIIa-158V/F Polymorphism Influences the Binding of IgG by Natural Killer Cell FcγRIIIa, Independently of the FcγRIIIa-48L/R/H Phenotype by Harry R.

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FcγRIIIa-158V/F Polymorphism Influences the Binding of IgG by Natural Killer Cell FcγRIIIa, Independently of the FcγRIIIa-48L/R/H Phenotype by Harry R. Koene, Marion Kleijer, Johan Algra, Dirk Roos, Albert E.G.Kr. von dem Borne, and Masja de Haas Blood Volume 90(3):1109-1114 August 1, 1997 ©1997 by American Society of Hematology

NlaIII restriction analysis of the 94-bp FcγRIIIA-specific fragment, containing the polymorphic nucleotide 559. NlaIII restriction analysis of the 94-bp FcγRIIIA-specific fragment, containing the polymorphic nucleotide 559. A 1.2-kb FcγRIIIA-specific fragment was amplified from genomic DNA, followed by a nested PCR. The sense primer of this nested PCR contained a mismatch that introduced a NlaIII restriction site only in the FcγRIIIA-158V (559G) allele. Homozygous FcγRIIIA-158FF fragments were not digested (lanes 1 through 3). Three bands (94 bp, 61 bp, and 33 bp) were visible in heterozygous individuals, whereas homozygous FcγRIIIA-158VV fragments were maximally digested (lanes 7 through 9). A 92-bp fragment of low intensity remained in homozygous 559G fragments (lanes 7 through 9). Harry R. Koene et al. Blood 1997;90:1109-1114 ©1997 by American Society of Hematology

FcγRIIIaNK-158V binds more IgG than does FcγRIIIaNK-158F. FcγRIIIaNK-158V binds more IgG than does FcγRIIIaNK-158F. IgG binding by NK cells from individuals either homozygous FcγRIIIA-158FF (▪) or homozygous FcγRIIIA-158VV (□) was compared, irrespective of the FcγRIIIa-48L/R/H genotype. At least three different donors of each genotype were tested. The level of cytophilic IgG and the binding of IgG1, IgG3, and IgG4 was significantly higher in NK cell from FcγRIIIA-158VV individuals (P < .05 in all cases). Harry R. Koene et al. Blood 1997;90:1109-1114 ©1997 by American Society of Hematology