Potential Role of a Mismatched HLA-Specific CTL Clone Developed Pre-Transplant in Graft Rejection following Cord Blood Transplantation Hiroto Narimatsu,

Slides:

Advertisements

Similar presentations

Galactomannan Antigen Enzyme-Linked Immunosorbent Assay for Diagnosis of Invasive Aspergillosis after Hematopoietic Stem Cell Transplantation Patrick.

Advertisements

Strategies for the Identification of T Cell–Recognized Tumor Antigens in Hematological Malignancies for Improved Graft-versus-Tumor Responses after Allogeneic Blood.

Increased Plasma Indoleamine 2,3-Dioxygenase Activity and Interferon-γ Levels Correlate with the Severity of Acute Graft-versus-Host Disease after Allogeneic.

Aurora-A kinase: a novel target of cellular immunotherapy for leukemia

In Vitro Evaluation of Graft-versus-Graft Alloreactivity as a Tool to Identify the Predominant Cord Blood Unit before Double Cord Blood Transplantation

Pegylated TNF-α Receptor Does Not Prevent Acute Graft-versus-Host Disease in the Dog Leukocyte Antigen-Nonidentical Unrelated Canine Model Scott S. Graves,

HLA-DPB1 Mismatching Results in the Generation of a Full Repertoire of HLA-DPB1- Specific CD4+ T Cell Responses Showing Immunogenicity of all HLA-DPB1.

Promiscuity of the AlloHLA-A2 Restricted T Cell Repertoire Hampers the Generation of Minor Histocompatibility Antigen-specific Cytotoxic T Cells across.

Targeting Cytomegalovirus-Infected Cells Using T Cells Armed with Anti-CD3 × Anti- CMV Bispecific Antibody Lawrence G. Lum, Mayur Ramesh, Archana Thakur,

CD8+ cytotoxic T lymphocytes isolated from allogeneic healthy donors recognize HLA class Ia/Ib–associated renal carcinoma antigens with ubiquitous or restricted.

Immunologic potential of donor lymphocytes expressing a suicide gene for early immune reconstitution after hematopoietic T-cell–depleted stem cell transplantation.

Human Leukocyte Antigen–DO Regulates Surface Presentation of Human Leukocyte Antigen Class II–Restricted Antigens on B Cell Malignancies Anita N. Kremer,

Cytomegalovirus-specific T cells are primed early after cord blood transplant but fail to control virus in vivo by Suzanne M. McGoldrick, Marie E. Bleakley,

Genome-wide Profiling in AML Patients Relapsing after Allogeneic Hematopoietic Cell Transplantation Miguel Waterhouse, Dietmar Pfeifer, Milena Pantic,

Combined CD4+ Donor Lymphocyte Infusion and Low-Dose Recombinant IL-2 Expand FOXP3+ Regulatory T Cells following Allogeneic Hematopoietic Stem Cell Transplantation

Adoptive Cellular Therapy using Cells Enriched for NKG2D+CD3+CD8+T Cells after Autologous Transplantation for Myeloma Kenneth R. Meehan, Laleh Talebian,

Identification of a Coordinated CD8 and CD4 T Cell Response Directed Against Mismatched HLA Class I Causing Severe Acute Graft-versus-Host Disease Avital.

Cytomegalovirus-Specific Cytotoxic T Lymphocytes Can Be Efficiently Expanded from Granulocyte Colony-Stimulating Factor–Mobilized Hemopoietic Progenitor.

Volume 138, Issue 4, Pages (April 2010)

Volume 6, Issue 2, Pages (February 1997)

Adoptive Therapy with T Cells/NK Cells

Major ABO Blood Group Mismatch Increases the Risk for Graft Failure after Unrelated Donor Hematopoietic Stem Cell Transplantation Mats Remberger, Emma.

Twenty Years of Unrelated Donor Hematopoietic Cell Transplantation for Adult Recipients Facilitated by the National Marrow Donor Program Chatchada Karanes,

Sabina Kersting, Leo F. Verdonck

Recovery of Varicella-Zoster Virus–Specific T Cell Immunity after T Cell–Depleted Allogeneic Transplantation Requires Symptomatic Virus Reactivation

Fatal Early-Onset Epstein-Barr Virus–Associated Posttransplantation Lymphoproliferative Disease after Successful Adult Dual-Unit Umbilical Cord Blood.

A Meta-Analysis of Unrelated Donor Umbilical Cord Blood Transplantation versus Unrelated Donor Bone Marrow Transplantation in Adult and Pediatric Patients

Detection of Microchimerism by Minor Histocompatibility Antigen HA-1 Allele-Specific Nested Polymerase Chain Reaction Brigitte Wieles, Jos Pool, Richard.

Cytomegalovirus Infection after Allogeneic Transplantation: Comparison of Cord Blood with Peripheral Blood and Marrow Graft Sources Christopher M. Walker,

Co-stimulated/Tc2 cells abrogate murine marrow graft rejection

Natural Killer Cell Killing of Acute Myelogenous Leukemia and Acute Lymphoblastic Leukemia Blasts by Killer Cell Immunoglobulin-Like Receptor–Negative.

Volume 18, Issue 4, Pages (April 2010)

Dynamic Detection of Anti–Human Leukocyte Antigen (HLA) Antibodies but not HLA-DP Loci Mismatches Can Predict Acute Graft-versus-Host Disease and Overall.

Induction of T-Cell Responses against Cutaneous T-Cell Lymphomas Ex Vivo by Autologous Dendritic Cells Transfected with Amplified Tumor mRNA Xiao Ni,

Sarah Nikiforow, Jerome Ritz

Molecular Monitoring of Minimal Residual Disease in the Peripheral Blood of Patients with Multiple Myeloma Mark Korthals, Nina Sehnke, Ralf Kronenwett,

HLA Haploidentical Stem Cell Transplant with Pretransplant Immunosuppression for Patients with Sickle Cell Disease Anna B. Pawlowska, Jerry C. Cheng,

B Cells and Transplantation: An Educational Resource

A comparison of gene transfer and antigen-loaded dendritic cells for the generation of CD4+ and CD8+ cytomegalovirus-specific T cells in HLA-A2+ and HLA-A2−

Jianbo Song, Danielle Mercer, Xiaofeng Hu, Henry Liu, Marilyn M. Li

Hematopoietic Engraftment in Recipients of Unrelated Donor Umbilical Cord Blood Is Affected by the CD34+ and CD8+ Cell Doses Seitaro Terakura, Eiichi.

Persistence of host dendritic cells after transplantation is associated with graft-versus- host disease Geoffrey W. Chan, Gullu Gorgun, Kenneth B. Miller,

Adverse Prognostic Factors for Morbidity and Mortality During Peripheral Blood Stem Cell Mobilization in Patients with Light Chain Amyloidosis Jason.

Early Vaccination with Tumor Lysate-Pulsed Dendritic Cells after Allogeneic Bone Marrow Transplantation Has Antitumor Effects Jeffrey S. Moyer, Gabriel.

HLA-Mismatched, Noninherited Maternal Antigen–Matched Unrelated Cord Blood Transplantations Have Superior Survival: How HLA Typing the Cord Blood Donor's.

Pauline Damien, David S. Allan

ASBMT and CBMTG Release Choosing Wisely BMT Recommendations

Galactomannan Antigen Enzyme-Linked Immunosorbent Assay for Diagnosis of Invasive Aspergillosis after Hematopoietic Stem Cell Transplantation Patrick.

What is quality in a transplant program?

Unrelated Stem Cell Transplantation after a Reduced Intensity Conditioning Regimen Containing High-Dose Thymoglobulin Leads to Controllable Graft-versus-Host.

Douglas E. Gladstone, Andrea A. Zachary, Ephraim J

Chimeric Antigen Receptor–Modified T Cells: Clinical Translation in Stem Cell Transplantation and Beyond Stanley R. Riddell, Michael C. Jensen, Carl.

Allogeneic Hematopoietic Stem Cell Transplantation in FLT3-ITD–Positive Acute Myelogenous Leukemia: The Role for FLT3 Tyrosine Kinase Inhibitors Post-

Potential Role of a Mismatched HLA-Specific CTL Clone Developed Pre-Transplant in Graft Rejection following Cord Blood Transplantation Hiroto Narimatsu,

Unrelated Donor Hematopoietic Cell Transplantation: Factors Associated with a Better HLA Match Jason Dehn, Mukta Arora, Stephen Spellman, Michelle Setterholm,

Blood and Marrow Transplant Handbook

Young Female Donors Do Not Increase the Risk of Graft-versus-Host Disease or Impact Overall Outcomes in Pediatric HLA-Matched Sibling Hematopoietic Stem.

T Cell–Depleted Related HLA-Mismatched Peripheral Blood Stem Cell Transplantation as Salvage Therapy for Graft Failure after Single Unit Unrelated Donor.

Long-Term Outcome after Bone Marrow Transplantation for Aplastic Anemia Using Cyclophosphamide and Total Lymphoid Irradiation as Conditioning Regimen

Low Serum Levels of Total Rabbit-IgG Is Associated with Acute Graft-Versus-Host Disease after Unrelated Donor Hematopoietic Stem Cell Transplantation:

Mammen Chandy Biology of Blood and Marrow Transplantation

Quantitative Assessment of T Cell Clonotypes in Human Acute Graft-versus-Host Disease Tissues Daisuke Koyama, Makoto Murata, Ryo Hanajiri, Tomohiro Akashi,

Molecular Therapy - Oncolytics

Impaired Interferon-Alpha Production by Plasmacytoid Dendritic Cells after Cord Blood Transplantation in Children: Implication for Post-transplantation.

Mary Eapen Biology of Blood and Marrow Transplantation

Volume 6, Issue 2, Pages (February 1997)

Futility of Relapsed Diffuse Large B Cell Lymphoma Transplantation?

Correlation of Infused CD3+CD8+ Cells with Single-Donor Dominance after Double-Unit Cord Blood Transplantation Filippo Milano, Shelly Heimfeld, Ted Gooley,

Optimal Donor Selection: Beyond HLA

CD137 Expression Identifies Leukemia Specific CTL after in Vitro Priming of Cord Blood T Cells Previously Expanded By CD3/CD28 Co-Stimulation Jeyaraj.

Presentation transcript:

Potential Role of a Mismatched HLA-Specific CTL Clone Developed Pre-Transplant in Graft Rejection following Cord Blood Transplantation Hiroto Narimatsu, Makoto Murata, Seitaro Terakura, Kyoko Sugimoto, Tomoki Naoe Biology of Blood and Marrow Transplantation Volume 14, Issue 4, Pages 397-402 (April 2008) DOI: 10.1016/j.bbmt.2008.01.001 Copyright © 2008 American Society for Blood and Marrow Transplantation Terms and Conditions

Figure 1 The N19D8 CTL clone recognizes HLA-B∗1501. (A) Transfection of HLA-B∗1501 cDNA into the B-LCL from the patient restored recognition by N19D8 CTL. The patient's B-LCL were transfected with a plasmid encoding HLA-B∗1501 cDNA and assayed as a target for N19D8 CTL. Cytotoxicity was determined using a chromium release assay. Lysis of the first-donor B-LCL (closed squares), second-donor B-LCL (open squares), patient B-LCL (triangles), and HLA-B∗1501-transfected patient B-LCL (circles) are shown as the mean of triplicate cultures at various E:T ratios. (B) HLA-B∗1501 cDNA stimulated interferon-γ production by N19D8 CTL. COS cells were transfected with a plasmid encoding HLA-B∗1501, B∗5603 (negative control), or B∗4403 (negative control) cDNA. COS transfectants were cocultured with N19D8 CTL, and interferon-γ production was measured in the supernatant, an enzyme-linked immunosorbent assay. Data is shown as the mean of triplicate determinations. Biology of Blood and Marrow Transplantation 2008 14, 397-402DOI: (10.1016/j.bbmt.2008.01.001) Copyright © 2008 American Society for Blood and Marrow Transplantation Terms and Conditions

Figure 2 Presence of the N19D8 clone in pretransplant peripheral blood from the patient. Detection of lymphocytes with the N19D8 clone-specific T cell receptor in pretransplant PBMCs. Nested PCR was performed on genomic DNA using a T cell receptor Vβ17-specific primer set for the first PCR. Genomic DNA was prepared from PBMCs obtained from the patient 1 month before the first CBT (pre PBMCs) and 3 or 9 months after the second CBT (post PBMCs). Genomic DNA from the N19D8 clone was used as a positive control. Genomic DNA prepared from PBMCs obtained from unrelated volunteers were used as negative controls. PCR to detect β-globin was used as an internal control in each assay. Biology of Blood and Marrow Transplantation 2008 14, 397-402DOI: (10.1016/j.bbmt.2008.01.001) Copyright © 2008 American Society for Blood and Marrow Transplantation Terms and Conditions

Figure 3 Presence of the N19D8 clone in pretransplant peripheral blood from the patient. The presence of HLA-B∗1501 microchimerism in pretransplant PBMCs. Lanes 1 and 6 are standard PCRs specific for HLA-B∗1501 on genomic DNA from pretransplant PBMCs from the patient and B∗1501-positive PBMCs from the patient's daughter (positive control), respectively. Lanes 2-3, 4-5, and 7-8 are nested PCR specific for HLA-B∗1501 on diluted (in parentheses) first PCR products from a posttransplant fingernail sample from the patient (negative control), pretransplant PBMCs from the patient, and PBMCs from the patient's daughter (positive control), respectively. PCR to detect complement factor 4 was used as an internal control in each assay. Biology of Blood and Marrow Transplantation 2008 14, 397-402DOI: (10.1016/j.bbmt.2008.01.001) Copyright © 2008 American Society for Blood and Marrow Transplantation Terms and Conditions