Pregnancies and live births after trophectoderm biopsy and preimplantation genetic testing of human blastocysts Steven J. McArthur, B.Sc., Don Leigh,

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Pregnancies and live births after trophectoderm biopsy and preimplantation genetic testing of human blastocysts Steven J. McArthur, B.Sc., Don Leigh, Ph.D., James T. Marshall, B.Appl.Sc., Kylie A. de Boer, Ph.D., Robert P.S. Jansen, M.D., C.R.E.I. Fertility and Sterility Volume 84, Issue 6, Pages 1628-1636 (December 2005) DOI: 10.1016/j.fertnstert.2005.05.063 Copyright © 2005 American Society for Reproductive Medicine Terms and Conditions

FIGURE 1 Biopsy of hatching blastocyst. For a video, see www.sydneyivf.com/videos. (A) Day-5 expanding blastocyst with trophectoderm herniating through an opening in the zona made 1 or 2 days earlier by microlaser. The embryo is secured by the holding pipette at the pole opposite to the opening. (B) Close-up of trophectoderm at the site for biopsy. (C) The fine biopsy pipette is brought onto the trophectoderm, (D) cells are drawn into the pipette, and (E) are teased free from the remaining trophectoderm, assisted by the laser (not shown) pulsed perpendicularly to the axis of the pipette, away from the embryo. (F) The biopsied blastocyst and the two- to nine-cell biopsy sample. McArthur. Blastocyst biopsy for PGD. Fertil Steril 2005. Fertility and Sterility 2005 84, 1628-1636DOI: (10.1016/j.fertnstert.2005.05.063) Copyright © 2005 American Society for Reproductive Medicine Terms and Conditions

FIGURE 2 Biopsy of fully hatched blastocyst. For a video, see www.sydneyivf.com/videos. (A) Day-6 fully hatched blastocyst. The embryo is secured by the holding the pipette at the inner cell mass. (B) Trophectoderm opposite to the inner cell mass is brought into the fine biopsy pipette. (C) The collapsing blastocyst is released from the holding pipette and (D) maneuvered to the rim of the pipette to detach the biopsy. (E) The blastocyst and the sample of trophectoderm immediately after biopsy. (F) The re-expanded blastocyst after completion of the biopsy procedure. McArthur. Blastocyst biopsy for PGD. Fertil Steril 2005. Fertility and Sterility 2005 84, 1628-1636DOI: (10.1016/j.fertnstert.2005.05.063) Copyright © 2005 American Society for Reproductive Medicine Terms and Conditions