Inhibition of ILK by QLT-0267 abrogates PAI-1 and MMP-2 induction by TGF-β1 in tubular epithelial cells. Inhibition of ILK by QLT-0267 abrogates PAI-1.

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Inhibition of ILK by QLT-0267 abrogates PAI-1 and MMP-2 induction by TGF-β1 in tubular epithelial cells. Inhibition of ILK by QLT-0267 abrogates PAI-1 and MMP-2 induction by TGF-β1 in tubular epithelial cells. (A and B) RT-PCR demonstrated a suppressed PAI-1 mRNA expression in HKC-8 cells by QLT-0267. (A) Representative RT-PCR assay. (B) Graphic presentation of PAI-1 mRNA expression in various groups. Data are means ± SEM of three experiments. *P < 0.05 versus controls; †P < 0.05 versus TGF-β1 alone. (C) Western blot demonstrates that QLT-0267 suppressed TGF- β1–induced PAI-1 expression in HKC-8 cells. (D and E) RT-PCR showed that QLT-0267 inhibited MMP-2 mRNA expression in HKC-8 cells. A representative RT-PCR assay (D) and graphic presentation of MMP-2 mRNA expression in various groups (E) are given. *P < 0.05 versus controls; †P < 0.05 versus TGF-β1 alone. (F) Zymographic analysis shows the proteolytic activity of MMP-2 in the supernatants of HKC-8 cells after various treatments. The location of MMP-2 is indicated. Yingjian Li et al. JASN 2009;20:1907-1918 ©2009 by American Society of Nephrology