The RD1-encoded antigen Rv3872 of Mycobacterium tuberculosis as a potential candidate for serodiagnosis of tuberculosis P. Mukherjee, M. Dutta, P. Datta,

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The RD1-encoded antigen Rv3872 of Mycobacterium tuberculosis as a potential candidate for serodiagnosis of tuberculosis P. Mukherjee, M. Dutta, P. Datta, A. Dasgupta, R. Pradhan, M. Pradhan, M. Kundu, J. Basu, P. Chakrabarti Clinical Microbiology and Infection Volume 13, Issue 2, Pages 146-152 (February 2007) DOI: 10.1111/j.1469-0691.2006.01660.x Copyright © 2007 European Society of Clinical Infectious Diseases Terms and Conditions

FIG. 1 Expression and purification of Mycobacterium tuberculosis proteins Rv3872, Rv3878, ESAT-6 and CFP-10. Coomassie blue-stained SDS gels showing (a) uninduced, (b) induced and (c) purified proteins. Protein marker sizes are indicated on the left side of each gel. Clinical Microbiology and Infection 2007 13, 146-152DOI: (10.1111/j.1469-0691.2006.01660.x) Copyright © 2007 European Society of Clinical Infectious Diseases Terms and Conditions

FIG. 2 Western blot of Mycobacterium tuberculosis proteins using human sera. Purified recombinant proteins (as indicated) were separated by SDS-PAGE, transferred electrophoretically to nitrocellulose membranes, and probed with human sera. Blots are representative of data obtained with healthy bacille Calmette–Guérin (BCG)-vaccinated individuals (lanes a and b) and pulmonary TB patients (lanes c–f). Clinical Microbiology and Infection 2007 13, 146-152DOI: (10.1111/j.1469-0691.2006.01660.x) Copyright © 2007 European Society of Clinical Infectious Diseases Terms and Conditions

FIG. 3 Antibody responses to recombinant (r)-Rv3872, r-Rv 3878, r-ESAT-6, r-CFP10 and a mixture of peptides (P8 and P9) derived from Rv3872. Levels of antibody response in pulmonary (group 1) and extrapulmonary (group 2) tuberculosis patients, as well as healthy individuals vaccinated with bacille Calmette–Guérin (group 3) were measured by ELISA. Each point represents one serum sample. The horizontal bar in each panel represents the cut-off value determined as mean absorbance plus 3 SD by use of negative control sera. Clinical Microbiology and Infection 2007 13, 146-152DOI: (10.1111/j.1469-0691.2006.01660.x) Copyright © 2007 European Society of Clinical Infectious Diseases Terms and Conditions

FIG. 4 Antibody response of tuberculosis (TB) patients and healthy individuals vaccinated with bacille Calmette–Guérin (BCG) to synthetic peptides. (a, c) Serum reactivity to individual synthetic peptides or a cocktail of P8 and P9 with sera from extra-pulmonary (a) and pulmonary (c) TB patients. (b) Serum reactivity to the peptides with sera from pulmonary TB patients and healthy, BCG-vaccinated individuals. (d) Serum reactivity to peptide cocktail (P8 and P9) with sera from pulmonary (group 1) and extra-pulmonary (group 2) TB patients, as well as from healthy, BCG-vaccinated individuals (group 3). Responses to all the peptides were plotted as absorbance at 490 nm (means ± SD). For (b), the differences in response between normal and patient sera were not significant for peptides P1–P6 and P11 (p >0.05). For (d), comparison was made with respect to sera from healthy BCG-vaccinated individuals (group 3). ***p <0.0001; **p <0.001; *p <0.01. Statistical analyses were performed using anova for (c) and Student's t-test for (d). Clinical Microbiology and Infection 2007 13, 146-152DOI: (10.1111/j.1469-0691.2006.01660.x) Copyright © 2007 European Society of Clinical Infectious Diseases Terms and Conditions