Zip and ZnT expression profiles of Zn-deficient middle-aged rat prostates resemble profiles of human prostate cancer and the relationship between miR-182.

Slides:

Advertisements

Similar presentations

Glioma-derived soluble factor(s) enhance IL-10 signaling.

Advertisements

From: Sonic Hedgehog Expression and Role in Healing Corneal Epithelium

Histogenic analysis confirms prostate cancer metastasis to lung.

Loss of Hdac3 impairs Ar signaling in mouse prostate tissues and has no effect on apoptosis Loss of Hdac3 impairs Ar signaling in mouse prostate tissues.

Nuclear Accumulation of Histone Deacetylase 4 (HDAC4) Coincides with the Loss of Androgen Sensitivity in Hormone Refractory Cancer of the Prostate K.

Comparison (θ) of the mean dinucleotide variability distribution for (A) genes with MAE vs. Comparison (θ) of the mean dinucleotide variability distribution.

Comparison (R) of the mean observed to expected dinucleotide distribution for (A) genes with MAE vs. Comparison (R) of the mean observed to expected dinucleotide.

RNAscope The Journal of Molecular Diagnostics

Light micrograph of adult rat brain DAB immunostained for Reelin (blue) and counterstained with neutral red for cells (red). Light micrograph of adult.

Altered cell migration factors and increased intestinal tumorigenesis in APC1638N/+ mice. Altered cell migration factors and increased intestinal tumorigenesis.

Establishment of a Zn-deficient middle-aged Sprague–Dawley rat model with prostatic Zn loss. Establishment of a Zn-deficient middle-aged Sprague–Dawley.

Untargeted metabolomics profiling by GC-TOF-MS reveals a human PCa-associated metabolic phenotype in Zn-deficient middle-aged Wistar-Unilever rat prostates.

Detection of CD38+IgG1+ memory B cells adjacent to contracted GCs

Volume 133, Issue 5, Pages e1 (November 2007)

Long-term decrease of IEC migration after 56Fe radiation relative to γ-rays. Long-term decrease of IEC migration after 56Fe radiation relative to γ-rays.

Heavy-ion radiation decreases expression of key cellular tight junction proteins and alters microtubule dynamics in mouse intestine. Heavy-ion radiation.

Klotho depletion increases MHC class II expression in the CP

Ilp6 and egr regulate epithelial homeostasis.

Klotho reduction causes macrophage invasion into the CP

Stem/progenitor-specific TF profiling identifies ligands of major pathways. Stem/progenitor-specific TF profiling identifies ligands of major pathways.

PD-1 and LAG-3 expression in MSI and MSS colorectal cancer specimens.

IHC staining of FFPE esophageal tissues in tissue microarrays (×20).

Volume 136, Issue 3, Pages (March 2009)

Intravenous delivery of reovirus to primary and secondary brain tumors

Figure 1 Reactivity of the patients' antibodies with rat brain and HEK cell-based assays Rat hippocampal dentate gyrus neuropils were stained with patient.

STED microscopy of MINOS

Mitofilin clusters are localized at the horizontal sides of mitochondria. Mitofilin clusters are localized at the horizontal sides of mitochondria. (A.

Candidates with a PVM localization.

Detection and tracking of individual SNAP-tagged proteins on the surface of living cells. Detection and tracking of individual SNAP-tagged proteins on.

Hdac3 deletion decreases AKT phosphorylation and tumor growth in Pten knockout prostate cancer Hdac3 deletion decreases AKT phosphorylation and tumor growth.

Association of AIM2, RIG‐I and NLRP3 inflammasomes with better survival in NPC patients.A.Overexpression of ASC, caspase‐1 and IL‐1β proteins as well as.

Prostate-specific LV-Cre/Luci delivery results in focal disease.

Apc deletion within the renal epithelium leads to rapid deletion of recombined cells and an upregulation of senescent markers.A and B. Kidneys imaged on.

Juxtapositioning of ARF5 with IRES-luc mRNA.

Haploinsufficiency at the Nkx3.1 locus

B cells infiltrate mouse and human pancreatic neoplasia and promote growth of KrasG12D-PDEC in vivo. B cells infiltrate mouse and human pancreatic neoplasia.

HDAC4N increases with Braak stage in CA2 hippocampal pyramidal cells.

Correlation of reovirus RNA/protein with proliferating tumor cells

Nox1 as a potential therapeutic target to inhibit matricellular-mediated endothelial senescence. Nox1 as a potential therapeutic target to inhibit matricellular-mediated.

ILK knockdown decreases mTOR signaling in PKD kidneys.

Differential secretion profiles of C. elegans TTR models.

Imaging intracellular endosymbiont E. coli by fluorescent microscopy.

Changes in c-Fos expression evoked by the gambling test under the standard or double-reward condition. c-Fos expression was analyzed immunohistochemically.

Volume 71, Issue 4, Pages (February 2007)

Fig. 4. smc3 regulates the expression of cx43 in regenerating fins.

Distinct requirements for Kita and Mitfa.

Fig. 1. Mitochondrial internalization in cardiomyocytes.

Detection of nuclear foci by FISH on frozen sections of diaphragm muscle prepared from 2-month-old DMSXL mice. Detection of nuclear foci by FISH on frozen.

Inflammasome is not activated in stable chronic obstructive pulmonary disease (COPD). a) Caspase-1 protein levels measured by ELISA and relative mRNA expression.

SIRT1 is downregulated in human gastric cancer (GC).

USP2a regulates MYC expression through the MDM2–p53 axis.

Histone crotonylation in kidney tubular cells.

Effect of M-cadherin RNAi on apoptosis in confluent C2C12 myoblasts.

Subnuclear localization of HA-tagged EBNA1 protein.

miR-9 regulation of Pax6 expression.

Ctip2 directly regulates Notch1 expression in differentiated cells.

Immunohistochemical analyses of SHH expression levels in nonmalignant prostate and adenocarcinoma tissues. Immunohistochemical analyses of SHH expression.

CCG regulates cellular localization and blocks target gene expression of MRTF. A, SK-Mel-147 cells were cotransfected with RhoC and the SRE.L reporter.

The expression of VCX3A and other CT antigens in pediatric HGG

Pancreatic cancer cell lines are sensitive to knockdown of outlier kinases. Pancreatic cancer cell lines are sensitive to knockdown of outlier kinases.

IL-13Rα2 expression is associated to human colon cancer progression and poor survival of patients with colorectal cancer. IL-13Rα2 expression is associated.

Fig. 2 IRF8 is expressed in CD68+ macrophages after SCI.

Activation of Ccl2–Ccr2 pathway is required for mammary carcinogenesis induced by Rb deficiency. Activation of Ccl2–Ccr2 pathway is required for mammary.

RUNX3 depletion induces cellular senescence and inflammatory cytokine expression in cells undergoing TGFβ-mediated EMT. A, Cells were transfected with.

Lineage-specific decline of E2F1 nuclear levels during oligodendrocyte differentiation. Lineage-specific decline of E2F1 nuclear levels during oligodendrocyte.

Expression of PCNA, K10, and K5 in skin lesions from Stat3+/−:HPV8 and Stat3+/+:HPV8 mice. Expression of PCNA, K10, and K5 in skin lesions from Stat3+/−:HPV8.

Coculture with U937 cells enhances DNMT1 expression in gastric cancer cells. Coculture with U937 cells enhances DNMT1 expression in gastric cancer cells.

Reduced β-catenin and cyclin D1 expression and increased apoptosis in lactating mammary gland epithelia of DDTg mice. Reduced β-catenin and cyclin D1 expression.

Determination of Per2 (left), MTNR1A (middle), and ERβ (right) protein expression levels in mammary tissues of rats sacrificed at ZT12. Determination of.

IHC staining of FFPE esophageal tissues in tissue microarrays (×20).

Presentation transcript:

Zip and ZnT expression profiles of Zn-deficient middle-aged rat prostates resemble profiles of human prostate cancer and the relationship between miR-182 up-regulation and ZIP1 mRNA/protein down-regulation. Zip and ZnT expression profiles of Zn-deficient middle-aged rat prostates resemble profiles of human prostate cancer and the relationship between miR-182 up-regulation and ZIP1 mRNA/protein down-regulation. (A and B) qPCR analyses of Zn importers (Zip 1–14; Zip12 is not detectable) (A) and Zn exporters (ZnT 1–10) (B) in prostates from Zn-deficient vs. Zn-sufficient middle-aged Wistar-Unilever rats (Oaz1 as normalizer, measurements performed in triplicate, n = 9–11 rats per cohort; two-sided t test). Asterisks denote Zip and ZnT expression similarly up- or down-regulated in human prostate cancer. (C) ISH cellular localization of miR-182 by mmu-miR-182 detection probe (double digoxigenin-labeled at the 5′ and 3′ ends) and IHC analysis of ZIP1 protein expression in FFPE prostate tissues of Zn-deficient vs. Zn-sufficient middle-aged rat prostates. Intense/frequent miR-182 ISH signal (blue) was detected in Zn-deficient middle-aged prostate (two representative samples are shown) vs. very weak and diffuse miR-182 ISH signals (blue) in Zn-sufficient middle-aged prostate (blue, 4-nitro-blue tetrazolium and 5-bromo-4-chloro-3′-indolyl phosphate counterstained by nuclear fast red). ZIP1 protein expression was diffuse and weak (brown staining, DAB) in representative ZN-deficient middle-aged rat prostate epithelia vs. strong ZIP1 expression (brown staining) in epithelial cells of a Zn-sufficient middle-aged rat prostate. n = 10 rats per group. (Scale bars: 50 μm in main panels and 25 μm in Insets.)‏ Louise Y. Fong et al. PNAS 2018;115:47:E11091-E11100 ©2018 by National Academy of Sciences