Comparison of survival and embryonic development in human oocytes cryopreserved by slow-freezing and vitrification Yun-Xia Cao, M.D., Ph.D., Qiong Xing,

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Presentation transcript:

Comparison of survival and embryonic development in human oocytes cryopreserved by slow-freezing and vitrification Yun-Xia Cao, M.D., Ph.D., Qiong Xing, M.D., Li Li, M.D., Lin Cong, M.D., Zhi-Guo Zhang, M.S., Zhao-Lian Wei, M.D., Ping Zhou, M.D. Fertility and Sterility Volume 92, Issue 4, Pages 1306-1311 (October 2009) DOI: 10.1016/j.fertnstert.2008.08.069 Copyright © 2009 American Society for Reproductive Medicine Terms and Conditions

Figure 1 Normal and abnormal images of spindle plate and chromosome alignment in the mature human oocytes cryopreserved by slow-freezing or vitrification. Oocytes were stained immunocytochemically with an anti-α-tubulin monoclonal antibody and fluorescein isothiocyanate to visualize the spindle (green) and counterstained with propidium iodide to visualize chromosomes (red). (a) The oocyte shows with normal spindle plate with chromosome alignment and the second polar body. (b) The same oocyte with (a) with different magnification. (c) The oocyte shows abnormal spindle plate. (d) The oocyte shows dispersed spindle plate and chromosomes. Scale bar indicates 30 μm. Fertility and Sterility 2009 92, 1306-1311DOI: (10.1016/j.fertnstert.2008.08.069) Copyright © 2009 American Society for Reproductive Medicine Terms and Conditions