Group Name Ecoli2 Group member name.

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Presentation transcript:

Group Name Ecoli2 Group member name

Isolates Sample name Country Collection date ec11-4623 France 2011-06-27 ec11-5537 2011-06-24 c227-11 Germany 2011-05-18 ec11-3798 2011 ec04-8351 2004

Result of species identification (KmerFinder) Strain Species acc. to Grad et al 2012 Predicted species Closest matching strain Template coverage ec11-4623 ec11-5537 c227-11 ec11-3798 ec04-8351

Representative KmerFinder result

Result of Multilocus Sequence Typing (MLST) Strain ST acc. to Grad et al 2012 Predicted ST ec11-4623 ec11-5537 c227-11 ec11-3798 ec04-8351

Representative Multilocus Sequence Typing result

Result of serotyping ec11-4623 ec11-5537 c227-11 ec11-3798 ec04-8351 Strain Serotype acc. to Grad et al 2012 Predicted serotype ec11-4623 ec11-5537 c227-11 ec11-3798 ec04-8351

Representative serotyping result

Result of antibiotics resistance analysis Isolate Ampicillin (blaZ, blaTem-1B) Third-generation cephalosporins (blaCTX-M-15) Streptomycin (strA, strB, aadA) Nalidixic acid (chromosomal mutations) Tetracycline (tetC, tetM, tetR, tetS, tetA) ec11-4623 ec11-5537 c227-11 ec11-3798 ec04-8351 Isolate Cotri-moxazole (dfr) Carbape-nems (blaNDM) Ciprofloxacin (chromosomal mutations) Chloram-phenicol (cat) Kanamycin (aph(3’)-II/aph(3’)-III) Gentamicin (aac(6')-aph(2'')) ec11-4623 ec11-5537 c227-11 ec11-3798 ec04-8351 How does it compare to the results presented in Grad et al 2012?

Virulence factors Isolate stx2 (stx2a variant) aggR pic stx1 eae hlyA astA papG dsbD ec11-4623 ec11-5537 c227-11 ec11-3798 ec04-8351 Indicate with , e.g., a “X”, if the gene was present or not Which pathovar does the outbreak isolates seem to belong to?

Result of plasmid analysis Isolate IncFII IncFIB IncI1 IncP IncQ1 IncB/O/K/Z ec11-4623 ec11-5537 c227-11 ec11-3798 ec04-8351 For each strain write which node the replicon was found on. If not found, leave the cell empty. Which plasmid is the blaCTX-M-15 gene on? Which plasmid are the aggR gene on?

Distance matrix made by NDtree and tree made with neighbour-joining

Distance matrix made by CSIPhylogeny and tree made with neighbour-joining