The impairment of reproduction in db/db mice is not mediated by intraovarian defective leptin signaling  Yuehui Zhang, M.D., Ph.D., Min Hu, M.D., Hongxia Ma, M.D., Ph.D., Junwei Qu, M.D., Ph.D., Yong Wang, Ph.D., Lihui Hou, M.D., Li Liu, M.D., Ph.D., Xiao-Ke Wu, M.D., Ph.D.  Fertility and Sterility  Volume 97, Issue 5, Pages 1183-1191 (May 2012) DOI: 10.1016/j.fertnstert.2012.01.126 Copyright © 2012 American Society for Reproductive Medicine Terms and Conditions

Figure 1 (A) Flowchart illustrating the experiment 1 design and the timing of the interventions. (B) Flowchart illustrating the experiment 2 design and the timing of the interventions. (C) Flowchart illustrating the experiment 3 design and the timing of the interventions. (D) Flowchart illustrating the experiment 1 ovarian transplantation and four genotypic mice models. (E) Flowchart illustrating the experiment 2 ovarian transplantation and two genotypic models. (F) Flowchart illustrating the experiment 3 ovarian transplantation and two genotypic models. Fertility and Sterility 2012 97, 1183-1191DOI: (10.1016/j.fertnstert.2012.01.126) Copyright © 2012 American Society for Reproductive Medicine Terms and Conditions

Figure 2 Experiment 1: Relative ovarian mRNA expressions of Star, Cyp17, Cyp19, and Ob-Rb at baseline in the WT Ov-db (n = 8), WT Ov-WT (n = 8), db Ov-WT (n = 8), and db Ov-db (n = 8). Values are mean ± SEM. ∗P<.01 vs. WT Ov-db; #P<.01 vs. WT Ov-WT; ★P<.01 vs. db Ov-WT (one-way ANOVA followed by Bonferroni post hoc test). Fertility and Sterility 2012 97, 1183-1191DOI: (10.1016/j.fertnstert.2012.01.126) Copyright © 2012 American Society for Reproductive Medicine Terms and Conditions

Figure 3 (A) Experiment 2: Ovarian morphology in WT Ov-WT/db mice and db Ov-WT/db mice. Panel a: db ovaries in WT mice. Panel b: WT ovaries in WT mice. Panel c: db ovaries in db mice. Panel d: WT ovaries in db mice (magnification, 4×). (B) Experiment 3: Relative ovarian mRNA expressions of Star, Cyp17, Cyp19, Ob-Rb, Jak2, Stat3, Pias3, and Socs3 at baseline and with IP hMG injections in the WT Ov-db/WT mice (n = 12). Values are mean ± SEM. ∗P<.01 vs. WT Ov-WT; #P<.01 vs. WT Ov-db or WT Ov-WT (one-way ANOVA followed by Bonferroni post hoc test). (C) Experiment 3: Relative ovarian mRNA expressions of Star, Cyp17, Cyp19, Ob-Rb, Jak2, Stat3, Pias3, and Socs3 at baseline and with IP hMG injections in db Ov-WT/WT (n = 7). Values are mean ± SEM. ∗P<.01 vs. saline group (one-way ANOVA followed by Bonferroni post hoc test). Fertility and Sterility 2012 97, 1183-1191DOI: (10.1016/j.fertnstert.2012.01.126) Copyright © 2012 American Society for Reproductive Medicine Terms and Conditions