A simple test for the detection of KPC and metallo-β-lactamase carbapenemase- producing Pseudomonas aeruginosa isolates with the use of meropenem disks.

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Presentation transcript:

A simple test for the detection of KPC and metallo-β-lactamase carbapenemase- producing Pseudomonas aeruginosa isolates with the use of meropenem disks supplemented with aminophenylboronic acid, dipicolinic acid and cloxacillin F. Pasteran, O. Veliz, D. Faccone, L. Guerriero, M. Rapoport, T. Mendez, A. Corso Clinical Microbiology and Infection Volume 17, Issue 9, Pages 1438-1441 (September 2011) DOI: 10.1111/j.1469-0691.2011.03585.x Copyright © 2011 European Society of Clinical Infectious Diseases Terms and Conditions

FIG. 1 Preliminary protocol for carbapenemase screening among Pseudomonas aeruginosa isolates. APB, 3-aminophenylboronic acid; AST, antimicrobial susceptibility test; CDT, combination disk test; DPA, dipicolinic acid; MBL, metallo-β-lactamase. The algorithm required a meropenem disk (10 µg) and meropenem disks supplemented with 600 µg of APB per disk, 1000 µg of DPA per disk and 3000 µg of cloxacillin per disk. Clinical Microbiology and Infection 2011 17, 1438-1441DOI: (10.1111/j.1469-0691.2011.03585.x) Copyright © 2011 European Society of Clinical Infectious Diseases Terms and Conditions