Ex. 10: Streak Plate Method of Isolation

Slides:

Advertisements

Similar presentations

Materials: Each pair needs (for controls and unknowns): 5 glucose broths (red cap, red liquid, durham tube) 4 lactose broths (white cap, red liquid, durham.

Advertisements

Lab 9. Overview  Instructor checks pre-labs. in lab notebook  Discussion of exam, sickle-cell disease write up, & the independent projects.  Basic Bacteria.

STREAKING AN AGAR PLATE FOR ISOLATIONOF BACTERIAL COLONIES BACTERIAL COLONIES.

Sterile technique When handling E. coli and other bacteria it is essential that the live cultures do not become contaminated with other bacteria or fungi.

ASEPTIC TECHNIQUE Removing inoculum from a broth culture

ASEPTIC TECHNIQUE Removing inoculum from a broth culture

Ex. 12: Chemical Antimicrobial Agents: Antibiotics Objectives ??

How to inoculate culture media

Microbiology Class Four Culture Characteristics. Day 4 Exp 2B, Isolation of pure cultures form SPD, Streak Plate Dilution, Technique. Procedure: page.

Lab 7 Goals and Objectives: Read the results: Gather all plates and tubes so we can discuss results together: ***Do NOT shake the FTM tubes!!!*** Your.

Pharmaceutical Microbiology

By: Kristin Canga Matt Hanchey B Nelson Stephanie Slagle

Culturing Yeast Cells on Media. Pre Lab Definitions: Petri Dish: A round, shallow dish used to grow bacteria. Culture: To grow living organisms in a prepared.

Ex. 7: Aseptic Technique – Inoculating Different Types of Media Key concepts: 1.Different types of bacterial cultures: broth, agar slant, and agar deep.

Lab 18 Goals and Objectives: Exercise 21: Enumeration Work as two pairs per group (max 5 groups) Both pairs use same Escherichia coli culture Use pre-poured.

General Microbiology Laboratory Sterile Technique & Pure Culture Concept.

LAB 4: ASEPTIC TECHNIQUE AND ISOLATION OF BACTERIA

Lab 8 Goals and Objectives: Do not disrupt BHI broths: need to see surface growth. Do not shake FTM tubes Make all Gram stain smears from broth & plates.

Supplemental instruction Chattahoochee Technical College

Recombinant DNA Bacterial Transformation Student Instructions Mid-Log Culture Prep.

Practical Part Microscopic Examination of Microorganisms Experiments Identification of MOs Different Staining Techniques.

Class Three February 7-9 Culture Characteristics

Lab 10- Colony isolation Mixed culture and unknown.

Results of Sources of Contamination. Fungi Bacteria.

Isolation of bacteria by dilution plating

Microbiology / Lab. 8. o Culture (Growth) Media I.What is a medium (plural media)? II.What is culture medium? III.What is meant by Inoculation of Media?

Pure Culture Techniques

Gather all plates and tubes so we can discuss results together:

Introduction In natural environments, microorganisms usually exist as mixed populations. However, if we are to study, characterize, and identify microorganisms,

Aseptic Technique This is how we grow bacteria and moulds without contaminating our cultures or ourselves. It is also called ‘sterile technique’. You must.

Lab 3 Goals and Objectives :

 Nutrients - C, H, O, N, S. P, K, Mg, Fe, Ca, Mn, and traces of Zn, Co, Cu, and Mo. These elements are found in the form of water, inorganic ions, small.

بسم الله الرحمن الرحيم 140 MICRO LAB 5 : PURIFICATION OF MICROORGANISM.

You’re never too old to streak!

Bacterial identification plating streaking how to inoculate how to observe.

*5 I’s Inoculation – Implanting Microbes into or upon culture media.

Safety Notes Bunsen burners> open flame

Lab Exercise 2 Culture Media. General Purpose Media: Nutrient Broth and Nutrient Agar.

Prof. Helen Poynton  Assistant Professor of Molecular Ecotoxicology, School for the Environment  Her Focus: studying genomics to understand the effects.

Aerosols Sneeze simulation method. Aerosols Suspension of a microbe containing particle Airborne pathogenic microorganisms Constitute a major hazard to.

Microbial Biotechnology Reem Alsharief Lab 3. General Methods of Isolation and selection of Microorganism Microbial isolation: To separate (a pure strain)

Isolation of microorganisms

By: 1. Teo Boon Boon 2. Khor Mei Ann 3. Ooi Shu YU.

Exercise 9: Aseptic Technique: Check results

Exercise 39: Oxidation and Fermentation Tests

Bacteria Cell Culture and Reproduction

Inoculation Incubation Isolation Inspection Identification 5/6/2018

Sterile Technique & Pure Culture Concept

Exercise 39: Oxidation and Fermentation Tests

Bacteria Cultures Biotechnology II.

Marine Biotechnology Lab

Aim: To prepare pure culture by streak plate method.

Tools of the Laboratory: Methods for Culturing of Microorganisms

PLASMID ISOLATION AND ANALYSIS Part I Overnight Culture Suspension

How does one calculate total magnification on your microscope?

Urine Culture Technique and the Importance of Selective and Differential Media for Gram-Negative Rods Vocabulary Coliforms Pure culture Types of culture.

By: Kristin Canga Matt Hanchey B Nelson Stephanie Slagle

You’re never too old to streak!

CULTURE METHODS Culture methods employed depend on the purpose for which they are intended. The indications for culture are: To isolate bacteria in pure.

pure culture isolation

Antibiotic sensitivity test

Aseptic Technique & Streaking for Isolation

Handling of Bacterial Cultures

Streaking an Agar Plate For

Ex. 16: Nitrate Respiration (Nitrate Reduction Test)

Culture media Pure culture techniques & Bacterial colony

Presentation transcript:

Ex. 10: Streak Plate Method of Isolation Objectives ?? Day 1 Materials needed per student: 1 nutrient agar (NA) plate Inoculating loop and Bunsen burner Cultures needed: From exercise 7: Your tube of mixed broth culture, isolated from your body. Each student works individually for this exercise!

Objective ?? Materials needed per student: Day 2 Materials needed per student: Streak plate from last session. One new NA plate, inoculating loop and Bunsen burner

The Quadrant Streak This technique is the most widely used method of obtaining pure (isolated) colonies from a mixed culture.

What is different here?

Movie Clip on How to Streak a Plate good luck