Preparation of Basic Growth Media

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Presentation transcript:

Preparation of Basic Growth Media Key Words and Concepts Macronutrients Micronutrients Fastidious organisms need lots of ____________ Solid vs. liquid media; complex vs. defined media

More Vocabulary Complex (undefined) vs. Synthetic (defined) media NA vs. NB TSA vs. TSB Complex (undefined) vs. Synthetic (defined) media Peptones

Always properly label your plates and tubes!

Today:

Making Media and Pouring Plates Prepare 100 ml NB media in 250 ml flask Weigh out nutrient broth powder for a 0.8% w/v solution. How much NA do you need ??? Warm the solution on the hot plate to dissolve but DO NOT boil. After removing 5 x 4ml to tubes, how much agar do you add to make a 1.5% agar suspension of the rest ???

Autoclave 121 ºC 15 psi 15 minutes

Good luck!