4-Methylumbelliferone blocks tail regeneration in a specific and time-dependent manner. 4-Methylumbelliferone blocks tail regeneration in a specific and.

Slides:

Advertisements

Similar presentations

Involvement of Wnt Signaling in Dermal Fibroblasts

Advertisements

Phenotypic analysis of spo73∆ mutant.

IL-1β stimulates CXCL5 and CXCL8 gene expression and protein secretion in A549 cells in a time- and dose-dependent manner. IL-1β stimulates CXCL5 and CXCL8.

Fate Restriction in the Growing and Regenerating Zebrafish Fin

Retinoic acid inhibits Th17 polarization and enhances FoxP3 expression through a Stat-3/Stat-5 independent signaling pathway by Kevin M. Elias, Arian Laurence,

by Cornelia Halin, Nadja E. Tobler, Benjamin Vigl, Lawrence F

Inhibition of ILK restores epithelial ZO-1 and E-cadherin and inhibits fibronectin and Snail1 expression after TGF-β1 treatment. Inhibition of ILK restores.

Impairment of Listeria protrusion formation by overexpression of the cytoplasmic tail of CD44. Impairment of Listeria protrusion formation by overexpression.

Naoki Hisamoto, Chun Li, Motoki Yoshida, Kunihiro Matsumoto

by Kelly A. McGowan, Wendy W. Pang, Rashmi Bhardwaj, Marcelina G

Sarita Sehra, Ana P. M. Serezani, Jesus A. Ocaña, Jeffrey B

Suppressive effect of CD39+CD73+ melanoma cells on T-cell proliferation, and reversion of this effect via treatment with the CD39-blocking antibody, CD39.

PGE2 signal via EP2 receptors evoked by a selective agonist enhances regeneration of injured articular cartilage S. Otsuka, M.D., T. Aoyama, M.D., Ph.D.,

Activin-βA Signaling Is Required for Zebrafish Fin Regeneration

Nuclear Arp3 mediates formation of TCR-induced nuclear actin filaments

Caroline W Beck, Bea Christen, Jonathan M.W Slack Developmental Cell

Sphingosine-1-phosphate signaling modulates terminal erythroid differentiation through the regulation of mitophagy Chong Yang, Michihiro Hashimoto, Quy.

Arc expression increases H4K12Ac levels.

Naoki Hisamoto, Chun Li, Motoki Yoshida, Kunihiro Matsumoto

Epithelial Cells in the Hair Follicle Bulge do not Contribute to Epidermal Regeneration after Glucocorticoid-Induced Cutaneous Atrophy Dmitry V. Chebotaev,

A Chemical Genetics Approach Reveals H,K-ATPase-Mediated Membrane Voltage Is Required for Planarian Head Regeneration Wendy S. Beane, Junji Morokuma,

Dpl-1 DP and efl-1 E2F Act with lin-35 Rb to Antagonize Ras Signaling in C. elegans Vulval Development Craig J. Ceol, H.Robert Horvitz Molecular Cell

Sarita Sehra, Ana P. M. Serezani, Jesus A. Ocaña, Jeffrey B

Time‐course analysis of NICD degradation.

Location, morphology and gene expression of skin CD8α+MHC II+ cells.

Clonally derived neurosphere efficiently contributes to spinal cord regeneration and reconstitutes the whole complement of spinal cord cell types. Clonally.

Fig. 8. In vitro effect of CMPs on adult DSS-treated stomach explants.

Wnt/β-catenin signaling regulates FGF signaling during fin regeneration. Wnt/β-catenin signaling regulates FGF signaling during fin regeneration. (A) fgf20a.

De novo WASP expression restores the defective MK differentiation of WASP-KO cells. De novo WASP expression restores the defective MK differentiation of.

Fig. 3. Characterization of unclassified cells (UCs).

IFN-γ and TNF-α cause an upregulation of Fas expression in human ESCs

Regulation of FoxB expression.

Apoptotic oocytes in the ovary-like gonadal tissue.

Cyclopamine treatment blocks Hh signalling in Xenopus.

RECQL4 is a MAP with a spindle function.

Apoptotic somatic cells in ovarian connective tissues.

Fig. 3. Exogenous folic acid rescues neural epithelial apical constriction and activation of non-muscle myosin upon Rho-kinase inhibition. Exogenous folic.

DRN and DRNL regulate STM expression via binding to a conserved promoter motif. DRN and DRNL regulate STM expression via binding to a conserved promoter.

Male and female Tg(fli1a:EGFP) blood vessel regeneration.

Lar function is not required for Stat92E accumulation in GSCs

Abnormal adherens junctions between hub cells and GSCs in Lar mutants.

Apc2 localization and centrosome orientation are altered in Lar mutant GSCs. (A-B′) Apc2 (white, A,B; red, A′,B′) localization in wild-type (A,A′) and.

Global cell proliferation replenishes epidermal cells.

Wrch-1 localizes to focal adhesions.

RECQL4 depletion or malfunction increases inter-kinetochore distance.

Fig. 3. MO-mediated smc3 knockdown results in reduced regenerate length, segment length and cell proliferation. MO-mediated smc3 knockdown results in reduced.

Hedgehog signaling plays a conserved role in inhibiting fat formation

A Bmp/Admp Regulatory Circuit Controls Maintenance and Regeneration of Dorsal- Ventral Polarity in Planarians Michael A. Gaviño, Peter W. Reddien Current.

Association of NM-HA and NM-GFP with SGs is transient.

BEZ235 induces MAPK signaling in a FOXO3A-dependent manner.

CD151 effects on mammary cell morphology and adhesion.

Fig. 6 Metarrestin treatment reduces pre-RNA synthesis and Pol I occupancy at rDNA without changing rDNA chromatin states. Metarrestin treatment reduces.

Ezrin phenotypes in the MVID intestine.

Effect of myosin Vb shRNA on ezrin phosphorylation and microvilli organization in Caco-2 cells. Effect of myosin Vb shRNA on ezrin phosphorylation and.

DHA induces apoptosis independently from caspase-8 and FADD in Jurkat T-lymphoma cells. DHA induces apoptosis independently from caspase-8 and FADD in.

Activating mutation of Ras is associated with CDCP1 expression in NSCLC. A, NSCLC cell lines with Ras + B-Raf mutations and wild-type Ras were examined.

Oncogenic Ras signaling activates SFKs and promotes CDCP1-dependent invasion in 3-dimensional (3D) collagen raft culture of HCK. A, HCK1T-E cells, HCK1T-E.

Induction of CDCP1 is regulated by Ras in NSCLC cells.

Figure 4 Cell count of selective immune cell subpopulations during alemtuzumab Cell count of selective immune cell subpopulations during alemtuzumab Absolute.

A, GP and GR epithelial tumor cells are equally responsive to gefitinib. A, GP and GR epithelial tumor cells are equally responsive to gefitinib. Equal.

Comparison of OPN production by mature DCs and activated macrophages.

GSDMD localizes to the plasma membrane and is processed during NET formation. GSDMD localizes to the plasma membrane and is processed during NET formation.

N-3 PUFAs promote endometrial cancer cell apoptosis in vitro and in vivo. n-3 PUFAs promote endometrial cancer cell apoptosis in vitro and in vivo. HEC-1-A.

CDCP1 links Ras and SFK signaling and regulates anoikis resistance, cell migration, and invasion in NSCLC cells. CDCP1 links Ras and SFK signaling and.

Ki-67 expression in M31- and H3-treated tumors (A) and respective Ki-67 labeling indices in the two groups of tumors (B). Ki-67 expression in M31- and.

NT157 treatment inhibits LNCaP xenograft growth and delays castration-resistant progression. NT157 treatment inhibits LNCaP xenograft growth and delays.

Lineage-specific decline of E2F1 nuclear levels during oligodendrocyte differentiation. Lineage-specific decline of E2F1 nuclear levels during oligodendrocyte.

Effect of MZ treatment on lung colony formation in an experimental metastasis. Effect of MZ treatment on lung colony formation in an experimental metastasis.

Figure 4. Histological features of defect healing 7 weeks after defect creation (Masson trichrome staining, scale bar=1 mm). Group 1 (A) showed notable.

Coculture with U937 cells enhances DNMT1 expression in gastric cancer cells. Coculture with U937 cells enhances DNMT1 expression in gastric cancer cells.

Presentation transcript:

4-Methylumbelliferone blocks tail regeneration in a specific and time-dependent manner. 4-Methylumbelliferone blocks tail regeneration in a specific and time-dependent manner. (A,B) Tadpoles at stage 41/42 (A) or 49/50 (B) were amputated and incubated during the first 24 or 48 hpa with 4-Mu, respectively. After removal of the chemical tails were allowed to regenerate and regeneration assessed by tail external appearance under the stereoscope. Regeneration percentage (white bars) and score of regeneration (black bars) are depicted. (C-F) Morphological appearance of regenerating tadpoles incubated with 4-Mu. (C) Control and (D) 4-Mu treated tadpoles were allowed to regenerate for 6 days and their external appearance was evaluated. (E) Control and (F) 4-Mu treated regenerating tails were fixed at 2 dpa and stained with Hematoxylin/Safranin. The terminal ampulla (asterisk), immature notochord (arrow) and mesenchymal cells (arrowhead) are indicated. (G) 4-Mu effect is specific. Amputated tails were incubated during the first day with 200 μM 4-Mu and equimolar amounts of UDP-GlcUA. (H) Semi-quantitative RT-PCR analysis of the expression levels of signaling pathway components at 2 dpa. Amputated tadpoles were incubated during the first 24 hours with or without 200 μM 4-Mu. (I) Time-dependent effect of 4-Mu. Tails from stage 41/42 tadpoles were amputated, incubated during different time windows with 150 μM 4-Mu and allowed to regenerate up to 6 dpa. The numbers on top of each bar correspond to the number of tadpoles analyzed for each experimental point. Scale bars: 500 μm in C,D; 200 μm in E,F. *P<0.05; **P<0.01; ***P<0.001. Esteban G. Contreras et al. Development 2009;136:2987-2996 © 2009. © 2009.