Industrial Production and Utilization of Enzymes

Slides:

Advertisements

Similar presentations

ENZYMES 1 ENZYMES Quick Quiz 1 Press the button when you’re sure… remember to take your time as you only get one chance!

Advertisements

Bioteknologi dalam Industri Pangan Nur Hidayat. Introduction Since the very beginning of human history, living systems and their extracts have been used.

Principles of Biology By Frank H. Osborne, Ph. D. Lab 4 - Enzymes and Digestion.

First Five  Turn in the Winter Break HW  Grab a packet from the podium  Spend the next 15 minutes reading and working on the packet.

USES OF ENZYMES.

Proteases break down proteins.

Enzymes in Industry Natural enzymes are used in many industrial processes (such as food processing, textiles) to: Speed up chemical reactions in the process.

Industrial Production of Enzyme. Biologically active enzymes may be extracted from any living organism: Of the hundred enzymes being used industrially,

Industrial Production and Utilization of Enzymes.

FOOD ENZYMES. What is an enzyme? A biological catalyst that promotes and speeds up a chemical reaction without itself being altered in the process. A.

Industrial enzyme production

Enzymes in Industry.

Enzymes Objective 2.01 Functions of Enzymes How Enzymes Work.

Biological catalysts Enzymes IGCSE Biology.

Protease Proteases break down proteins. Baby food: As babies can’t digest solid food, using protease enzymes makes it easier for a baby’s digestive system.

Microbial Biotechnology Microorganisms – Organisms that are too small to be seen without a microscope – Include: bacteria, fungi, protozoa, microalgae,

Enzymes and the catalase lab Chapter 2 – Biochemistry You’ve had most of this material; today’s info is mostly review (paraphrase what’s new or important.

Biotechnology – Biotechnological techniques 1.Use of micro-organisms 2.Industrial production of enzymes 3.Tissue cultures.

IB Enzymes in industry © Oxford University Press 2011 Enzymes in industry.

Catalase Sources - plant tissues e.g. potato tuber, apple animal tissues e.g. liver, muscle hydrogen peroxide  water + oxygen Speed up the.

Enzyme Grade 8 Year

Industrial Uses of Enzymes Washing powders food manufacture Drink manufacture.

Warm update 10/1 Define these words Chemical reaction Reactant Product Activation energy Catalyst Enzymes Substrate.

Beginnings and Endings Answers BeginningsEndings Cell division is essential (needed) for growth and repair During cell division the parent cell divides.

Useful Enzymes LO: To know that enzymes can be very useful, and to be able to explain the uses of enzymes in the home and industry.

Beneficial Uses of Microorganisms. Objective Criteria for the microbes Classification of microbes, example, uses.

Go through this presentation.

The Molecules of Life Chapter 5. Carbon Bonding Organic Compounds –Any compound that has Carbon in it Inorganic Compounds –Compound that does not contain.

This PowerPoint file contains the Plenary for this chapter in Biology For You. Most of these slides are paired with a corresponding slide in the PowerPoint.

Application of enzymes. Why use enzymes? Properties of enzymes: Very sensitive – can measure tiny concentrations of substances Specific – will only respond.

Uses of Enzymes in the Home and in Industry

Biology Respiration.

© SSER Ltd.. Enzyme technology is concerned with the application of enzymes as tools of industry, agriculture and medicine Enzymes are biological catalysts.

Prepared by, SEETHA U R.  An enzyme is a biocatalyst which accelerates biological reactions  It includes the pure enzyme,crude cell extract,viable plant.

Application of enzymes Specification ref: Text book ref:- Chapter 7 Page 113.

Chitin contents of different organisms Fungi 5-60% Worms 20-38% Squid 3-20% Scorpions 30% Spiders.

Biological catalysts Enzymes IGCSE Biology (Cambridge)

Review Question One category of organic compounds contains molecules composed of long hydrocarbon chains. The hydrocarbon chains may be saturated or unsaturated.

Biological catalysts Enzymes.

Biological catalysts Enzymes IGCSE Biology (Cambridge)

Biological catalysts Enzymes.

Proteins breaks down proteins into fatty acids and glycerol in stomach

Catalysts speed up the rate of the reaction.

Enzymes: Biological Catalysts

Unit 3: Biochemistry Part 1 Review

Catalysts speed up the rate of the reaction.

Biological catalysts Enzymes.

Enzymes in Germination

Which molecule does each of these digest?

Functions of Enzymes How Enzymes Work

Food Enzymes: Friend or Foe? A Great Teaching Topic

Which molecule does each of these “digest”

Metabolism cont’d.

Organic Compounds Chapter 6 section 3.

National 4/5 Biology Properties of enzymes.

Practical General Biology “ A” Slides

Application of Enzymes in Industry By Manisha A. Dhotre

Industrial Uses of Enzymes

Biological catalysts Enzymes IGCSE Biology (Cambridge)

INTRODUCTION TO SOLID STATE FERMENTATION AND SUBMERGED FERMENTATION

Biology & biotechnology department

DR . Hanan M. A. Abdel Razek Biotechnology.

Insilico analysis of cellulolytic fungi and their improvement through protoplast fusion for waste management.

Presentation transcript:

Industrial Production and Utilization of Enzymes

Enzyme Production

Cost of Purification The effect of number of steps on the yield and costs in a typical enzyme purification process. For example, Step Specific activity Unit/mg Cost per weight ($/mg) Cost per activity ($/unit) 1 1.00 3 4 1.47 2 9 19 2.13 27 83 3.08 81 358 4.92 5 243 1536 6.32

Sources of Enzymes Biologically active enzymes may be extracted from any living organism: Of the hundred enzymes being used industrially, - over a half are from fungi - over a third are from bacteria with the remainder divided between animal (8%) and plant (4%) sources .

Sources f Enzymes Microbes are preferred to plants and animals as sources of enzymes because: They are generally cheaper to produce. Their enzyme contents are more predictable and controllable. - Plant and animal tissues contain more potentially harmful materials than microbes, including phenolic compounds (from plants).

E: extracellular enzyme; I: intracellular enzyme Fungal Enzymes Enzyme EC Sources Application a-Amylase 3.2.1.1 Aspergillus E Baking Catalase 1.11.1.6 I Food Cellulase 3.2.1.4 Trichoderma Waste Dextranase 3.2.1.11 Penicillium Glucose oxidase 1.1.3.4 Lactase 3.2.1.23 Dairy Lipase 3.1.1.3 Rhizopus Rennet 3.4.23.6 Mucor miehei Cheese Pectinase 3.2.1.15 Drinks Protease Catalase:catalyzes the decomposition of hydrogen peroxide to water and oxygen. E: extracellular enzyme; I: intracellular enzyme

Bacterial Enzymes a-Amylase 3.2.1.1 Bacillus E Starch b-Amylase Sources Application a-Amylase 3.2.1.1 Bacillus E Starch b-Amylase 3.2.1.2 Asparaginase 3.5.1.1 Escherichia coli I Health Glucose isomerase 5.3.1.5 Fructose syrup Penicillin amidase 3.5.1.11 Pharmaceutical Protease 3.4.21.14 Detergent Asparaginase:(EC 3.5.1.1) is an enzyme that catalyzes the hydrolysis of asparagine to aspartic acid. Penicillin amidase: Sakaguchi and Murao1 reported on the presence of an enzyme in the mycelium of Penicillium chrysogenum and Aspergillus oryzae which would split penicillin G (I) into phenylacetic acid (II) and 'penicin' (III) :