Endothelin Receptor Antagonism–Based Treatment for Scleroderma Renal Crisis  Hassane Izzedine, MD, PhD, Philippe Rouvier, MD, Gilbert Deray, MD  American Journal of Kidney Diseases  Volume 62, Issue 2, Pages 394-395 (August 2013) DOI: 10.1053/j.ajkd.2013.04.016 Copyright © 2013 National Kidney Foundation, Inc. Terms and Conditions

Figure 1 Detection of endothelin 1 by immunoperoxidase staining of kidney tissue from (A, B) the case and (C, D) a healthy control. (A, C) Podocytes and (B, D) endothelial cells of an interlobular artery are shown, revealing cytoplasmic endothelin 1 (ET-1) in the case. Tissue was Bouin fixed and paraffin embedded; ET-1 antibody (catalog no. sc-21625; Santa Cruz Biotechnology Inc) was used at a dilution of 1/700; diaminobenzidine-based detection at pH 6). American Journal of Kidney Diseases 2013 62, 394-395DOI: (10.1053/j.ajkd.2013.04.016) Copyright © 2013 National Kidney Foundation, Inc. Terms and Conditions