Characterization of Aurora A and Its Impact on the Effect of Cisplatin-Based Chemotherapy in Patients with Non–Small Cell Lung Cancer Peng Kuang, Zuhua.

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Characterization of Aurora A and Its Impact on the Effect of Cisplatin-Based Chemotherapy in Patients with Non–Small Cell Lung Cancer Peng Kuang, Zuhua Chen, JiaYuan Wang, Zhentao Liu, Jingyuan Wang, Jing Gao, Lin Shen Translational Oncology Volume 10, Issue 3, Pages 367-377 (June 2017) DOI: 10.1016/j.tranon.2017.02.010 Copyright © 2017 The Authors Terms and Conditions

Figure 1 Aurora A expression in human NSCLC and adjacent normal tissue. (A) Aurora A was detected and overexpressed in the NSCLC tissue (100 ×). (B) Adjacent normal tissue showed nearly negative expression of Aurora A (100 ×). (A’ and B′) The higher magnification (200×) from the area of the box in A and B, respectively. Translational Oncology 2017 10, 367-377DOI: (10.1016/j.tranon.2017.02.010) Copyright © 2017 The Authors Terms and Conditions

Figure 2 Aurora A expression in human NSCLC and adjacent normal tissue. (A) Western blot analysis of Aurora A expression in representative primary NSCLC tissue (T) and adjacent normal tissue (N). (B) The mRNA results were consistent with protein results. Translational Oncology 2017 10, 367-377DOI: (10.1016/j.tranon.2017.02.010) Copyright © 2017 The Authors Terms and Conditions

Figure 3 ROC curves analysis of Aurora A cutoff score in the training set. (A) Aurora A cutoff point for OS in the training set. (B) Aurora A cutoff point for DFS in the training set. At each immunohistochemical score, the sensitivity and specificity for the outcome being studied were plotted, thus generating an ROC curve. Aurora A cutoff score for OS and DFS was 3.8 and 3.5, respectively. Translational Oncology 2017 10, 367-377DOI: (10.1016/j.tranon.2017.02.010) Copyright © 2017 The Authors Terms and Conditions

Figure 4 Kaplan-Meier survival analysis of Aurora A expression in overall patients. Patients with higher Aurora A expression acquired an inferior (A) OS and (B) DFS in overall patients. The median duration of OS and DFS for patients with high and low expression of Aurora A was 24.6 versus 78.8 months (P<.001) and 13.4 versus 56.8 months (P=.02), respectively. Translational Oncology 2017 10, 367-377DOI: (10.1016/j.tranon.2017.02.010) Copyright © 2017 The Authors Terms and Conditions

Figure 5 Kaplan-Meier survival analysis of Aurora A expression in subsets of NSCLC patients with stage I, II, and III disease (log-rank test). Probability of (A) OS and (B) DFS of patients with stage I NSCLC in the overall patients: low expression, n=67; high expression, n=27. Probability of (C) OS and (D) DFS of stage II patients with NSCLC in the overall patients: low expression, n=39; high expression, n=30. Probability of (E) OS and (F) DFS of patients with stage III NSCLC in the overall patients: low expression, n=29; high expression, n=71. Translational Oncology 2017 10, 367-377DOI: (10.1016/j.tranon.2017.02.010) Copyright © 2017 The Authors Terms and Conditions

Figure 6 (A) Protein levels of Aurora A in multiple lung cancer cell lines. Cell lysates were prepared from indicated cell lines, followed by Western blotting with equal amount protein loaded, using indicated antibodies. (B) Overexpression of Aurora A induced cisplatin resistance in lung cancers, whereas Aurora A knockdown sensitizes lung cancer cells to cisplatin-induced apoptosis. (C) Overexpression of Aurora A contributed to increased Snail and Slug with decreasing E-cad, which indicated EMT process. However, knockdown of Aurora A upregulated E-cad but downregulated Snail and Slug, which inhibited EMT process. Translational Oncology 2017 10, 367-377DOI: (10.1016/j.tranon.2017.02.010) Copyright © 2017 The Authors Terms and Conditions