Chondroitin-4-sulphate inhibits NF-kB translocation and caspase activation in collagen- induced arthritis in mice G.M. Campo, Ph.D., A. Avenoso, Ph.D.,

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General Information Osteoarthritis and Cartilage

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Chondroitin-4-sulphate inhibits NF-kB translocation and caspase activation in collagen- induced arthritis in mice G.M. Campo, Ph.D., A. Avenoso, Ph.D., S. Campo, Ph.D., A. D'Ascola, Ph.D., P. Traina, Ph.D., A. Calatroni, Ph.D. Osteoarthritis and Cartilage Volume 16, Issue 12, Pages 1474-1483 (December 2008) DOI: 10.1016/j.joca.2008.04.002 Copyright © 2008 Osteoarthritis Research Society International Terms and Conditions

Fig. 1 Effect of C4S treatment on the development (A) and progression (B) of arthritis at the end of experiment. Evaluation was performed at the end of the experiment (day 60). Values are the mean±S.D. of seven experiments. ○P<0.001 vs Control; *P<0.005 and **P<0.001 vs CIA. Osteoarthritis and Cartilage 2008 16, 1474-1483DOI: (10.1016/j.joca.2008.04.002) Copyright © 2008 Osteoarthritis Research Society International Terms and Conditions

Fig. 2 Effect of C4S treatment on the articular cartilage NF-kB p50/65 transcription factor DNA binding activity (A) and IkB-α protein degradation (B) in joints of mice with CIA. In panel A white bars represent the p50 subunit, black bars represent the p65 subunit. Values are the mean±S.D. of seven experiments and are expressed as OD at λ 450nm/mg protein of nuclear extract (A) and as OD measured at λ 450nm/mg protein (B). ○P<0.001 vs Control; *P<0.01 and **P<0.001 vs CIA. Osteoarthritis and Cartilage 2008 16, 1474-1483DOI: (10.1016/j.joca.2008.04.002) Copyright © 2008 Osteoarthritis Research Society International Terms and Conditions

Fig. 3 Effect of C4S treatment on the articular cartilage MMP-13 mRNA expression (A) and related protein production (B, C) in joints of mice with CIA. Values are the mean±S.D. of seven experiments and are expressed as the n-fold increase with respect to the Control (A) and as both densitometric analysis (C) and Western blot analysis (B) for the MMP-13 protein levels. ○P<0.001 vs Control; *P<0.005 and **P<0.001 vs CIA. Osteoarthritis and Cartilage 2008 16, 1474-1483DOI: (10.1016/j.joca.2008.04.002) Copyright © 2008 Osteoarthritis Research Society International Terms and Conditions

Fig. 4 Effect of C4S treatment on the articular cartilage caspase-3 mRNA expression (A) and related protein production (B, C) in joints of mice with CIA. Values are the mean±S.D. of seven experiments and are expressed as the n-fold increase with respect to the Control (A) and as both densitometric analysis (C) and Western blot analysis (B) for the caspase-3 protein levels. ○P<0.001 vs Control; *P<0.05, **P<0.005 and ***P<0.001 vs CIA. Osteoarthritis and Cartilage 2008 16, 1474-1483DOI: (10.1016/j.joca.2008.04.002) Copyright © 2008 Osteoarthritis Research Society International Terms and Conditions

Fig. 5 Effect of C4S treatment on the articular cartilage caspase-7 mRNA expression (A) and related protein production (B, C) in joints of mice with CIA. Values are the mean±S.D. of seven experiments and are expressed as the n-fold increase with respect to the Control (A) and as both densitometric analysis (C) and Western blot analysis (B) for the caspase-7 protein levels. ○P<0.001 vs Control; *P<0.05, **P<0.005 and ***P<0.001 vs CIA. Osteoarthritis and Cartilage 2008 16, 1474-1483DOI: (10.1016/j.joca.2008.04.002) Copyright © 2008 Osteoarthritis Research Society International Terms and Conditions

Fig. 6 Effect of C4S on matrix proteoglycan loss and erosion in joint cartilage. C, cartilage; F, femur; JS, joint space. Safranin O 150×. Osteoarthritis and Cartilage 2008 16, 1474-1483DOI: (10.1016/j.joca.2008.04.002) Copyright © 2008 Osteoarthritis Research Society International Terms and Conditions