Clonal expansion and TCR-independent differentiation shape the HIV-specific CD8+ effector-memory T-cell repertoire in vivo by Dirk Meyer-Olson, Brenna.

Slides:



Advertisements
Similar presentations
Novel Approaches: Treatment and HIV Pathogenesis L. Trautmann, Ph.D. VGTI Florida.
Advertisements

Differentiation stage determines pathologic and protective allergen-specific CD4+ T-cell outcomes during specific immunotherapy  Erik Wambre, PhD, Jonathan.
Volume 66, Issue 6, Pages (June 2013)
High-dimensional analysis of lymphoid CD4+ T cells identified distinct TFH cell subsets in HIV+ patients and HCs. High-dimensional analysis of lymphoid.
How I treat LGL leukemia
SIV-specific CD8+ T cells express high levels of PD1 and cytokines but have impaired proliferative capacity in acute and chronic SIVmac251 infection by.
TCR-MHC-peptide(s): in vivo veritas
Selective expansion of polyfunctional pathogen-specific CD4+ T cells in HIV-1–infected patients with immune reconstitution inflammatory syndrome by Yolanda.
Tissue Homing and Persistence of Defined Antigen-Specific CD8+ Tumor-Reactive T- Cell Clones in Long-Term Melanoma Survivors  Frédérique-Anne Le Gal, Valérie.
Blood T-cell receptor diversity decreases during the course of HIV infection, but the potential for a diverse repertoire persists by Paul D. Baum, Jennifer.
Sustained Interactions between T Cell Receptors and Antigens Promote the Differentiation of CD4+ Memory T Cells  Chulwoo Kim, Theodore Wilson, Kael F.
CD158k Is a Reliable Marker for Diagnosis of Sézary Syndrome and Reveals an Unprecedented Heterogeneity of Circulating Malignant Cells  Hélène Moins-Teisserenc,
Costimulatory and Coinhibitory Receptor Pathways in Infectious Disease
Emergence of muscle and neural hematopoiesis in humans
Long-term expansion of effector/memory Vδ2− γδ T cells is a specific blood signature of CMV infection by Vincent Pitard, David Roumanes, Xavier Lafarge,
by Jason M. Brenchley, Mirko Paiardini, Kenneth S. Knox, Ava I
Volume 69, Issue 3, Pages (September 2018)
CMV reactivation drives posttransplant T-cell reconstitution and results in defects in the underlying TCRβ repertoire by Yvonne Suessmuth, Rithun Mukherjee,
Volume 27, Issue 4, Pages (October 2007)
Volume 40, Issue 6, Pages (June 2014)
Impact of clonal competition for peptide-MHC complexes on the CD8+ T-cell repertoire selection in a persistent viral infection by Katherine K. Wynn, Zara.
Expansion of CD7low and CD7negative CD8 T-cell effector subsets in HIV-1 infection: correlation with antigenic load and reversion by antiretroviral treatment.
The transcriptional program of terminal granulocytic differentiation
FcRL6, a new ITIM-bearing receptor on cytolytic cells, is broadly expressed by lymphocytes following HIV-1 infection by Timothy J. Wilson, Rachel M. Presti,
by Vladia Monsurrò, Ena Wang, Yoshisha Yamano, Stephen A
Functional leukemia-associated antigen-specific memory CD8+ T cells exist in healthy individuals and in patients with chronic myelogenous leukemia before.
Volume 38, Issue 2, Pages (February 2013)
J. Joseph Melenhorst, Phillip Scheinberg, Ann Williams, David R
Viral load and CD4+ T cells in CD8+ T cell–depleted YU-2–infected MISTRG mice. Viral load and CD4+ T cells in CD8+ T cell–depleted YU-2–infected MISTRG.
Changes in splenic CD4+ and CD8+ T cell subsets during acute and chronic HIV infection in MISTRG mice. Changes in splenic CD4+ and CD8+ T cell subsets.
Volume 134, Issue 7, Pages e2 (June 2008)
Jug r 2–reactive CD4+ T cells have a dominant immune role in walnut allergy  Luis Diego Archila, MSc, David Jeong, MD, Mariona Pascal, PhD, Joan Bartra,
Volume 21, Issue 12, Pages (December 2017)
T-cell receptor diversity is selectively skewed in T-cell populations of patients with Wiskott-Aldrich syndrome  Junfeng Wu, MD, Dawei Liu, MS, Wenwei.
Volume 23, Issue 10, Pages (October 2015)
Volume 35, Issue 5, Pages (November 2011)
Peter A. Savage, Mark M. Davis  Immunity 
TCF1 expression marks self-renewing human CD8+ T cells
Volume 134, Issue 7, Pages e2 (June 2008)
Tissue Homing and Persistence of Defined Antigen-Specific CD8+ Tumor-Reactive T- Cell Clones in Long-Term Melanoma Survivors  Frédérique-Anne Le Gal, Valérie.
Volume 48, Issue 2, Pages e7 (February 2018)
Volume 38, Issue 1, Pages (January 2013)
Protective Immunity Transferred by Infusion of Cytomegalovirus-Specific CD8+ T Cells within Donor Grafts: Its Associations with Cytomegalovirus Reactivation.
TCR Specificity Dictates CD94/NKG2A Expression by Human CTL
Induction of WT1-specific human CD8+ T cells from human HSCs in HLA class I Tg NOD/SCID/IL2rgKO mice by Yuho Najima, Mariko Tomizawa-Murasawa, Yoriko Saito,
Cécile Bouneaud, Philippe Kourilsky, Philippe Bousso  Immunity 
Louise J. McHeyzer-Williams, Michael G. McHeyzer-Williams  Immunity 
Expansion of cytolytic CD4+CD28− T cells in end-stage renal disease
Costimulatory and Coinhibitory Receptor Pathways in Infectious Disease
Functional analysis of clinical response to low-dose IL-2 in patients with refractory chronic graft-versus-host disease by Jennifer S. Whangbo, Haesook.
Differentiation stage determines pathologic and protective allergen-specific CD4+ T-cell outcomes during specific immunotherapy  Erik Wambre, PhD, Jonathan.
Progression of mycosis fungoides occurs through divergence of tumor immunophenotype by differential expression of HLA-DR by Duncan Murray, Jack Luke McMurray,
Effects of a Single Escape Mutation on T Cell and HIV-1 Co-adaptation
Fig. 2 CD32+ cells have a distinctively different phenotype compared to CD32− cells. CD32+ cells have a distinctively different phenotype compared to CD32−
CD4-TEMRA cells show marked clonal expansion.
Donor and recipient BAL T cells are phenotypically and functionally memory T cells. Donor and recipient BAL T cells are phenotypically and functionally.
Volume 38, Issue 6, Pages (June 2013)
Flow cytometry of subcutaneous adipose tissue stromovascular cells.
IL-33, IL-25, and TSLP induce a distinct phenotypic and activation profile in human type 2 innate lymphoid cells by Ana Camelo, Guglielmo Rosignoli, Yoichiro.
Peptides identified on monocyte-derived dendritic cells: a marker for clinical immunogenicity to FVIII products by Wojciech Jankowski, Yara Park, Joseph.
CD4-TEMRA cells are heterogeneous across donors.
CD4+CLA+CD103+ T cells from human blood and skin share a transcriptional profile. CD4+CLA+CD103+ T cells from human blood and skin share a transcriptional.
CD4+CLA+CD103+ T cells in skin and blood are clonally related.
Shared phenotype of CD4+CLA+CD103+ T cells from human blood and skin.
MR1Ts recognized by the hpMR1+EC tetramer are more likely to be TRAV1-2−. MR1Ts recognized by the hpMR1+EC tetramer are more likely to be TRAV1-2−. PBMCs.
Volume 9, Issue 2, Pages (August 1998)
FCM and TCR γ gene rearrangement analysis in LGL leukemia.
A phenotypically and functionally distinct human TH2 cell subpopulation is associated with allergic disorders by Erik Wambre, Veronique Bajzik, Jonathan.
CD4-CTL effectors share TCR clonotypes with CD4-CTL precursors.
How to establish the diagnosis of LGL leukemia.
Presentation transcript:

Clonal expansion and TCR-independent differentiation shape the HIV-specific CD8+ effector-memory T-cell repertoire in vivo by Dirk Meyer-Olson, Brenna C. Simons, Joseph A. Conrad, Rita M. Smith, Louise Barnett, Shelly L. Lorey, Coley B. Duncan, Ramesh Ramalingam, and Spyros A. Kalams Blood Volume 116(3):396-405 July 22, 2010 ©2010 by American Society of Hematology

Expansions of HIV-specific TemRA phenotype cells are present to various degrees in chronic infection. Expansions of HIV-specific TemRA phenotype cells are present to various degrees in chronic infection. (A) Representative staining of CCR7 and CD45RO on CD8+ T cells in 2 representative HIV-seropositive persons. (B) Identification of the HIV-1-tetramer+ cells (red) and their corresponding Tmem phenotype (overlay: red tet+, black CD8+ T cells). (C) Comparison of effector-memory populations (paired t test) on CD8+ T cells and on HIV-1-tetramer+ T cells. Dirk Meyer-Olson et al. Blood 2010;116:396-405 ©2010 by American Society of Hematology

Analysis of effector-memory clonotype composition on HIV-specific effector-memory subsets. Analysis of effector-memory clonotype composition on HIV-specific effector-memory subsets. (A) Purification of B57-KF11 tetramer+ CD8+ T cells based on the expression of CCR7 and CD45RO in subject 10076. (B) Sequence analysis of the purified Tmem populations. (C) Analysis of the CCR7/CD45RO expression on B57-KF11 tetramer+ CD8+ T-cell expression TRBV2. Dirk Meyer-Olson et al. Blood 2010;116:396-405 ©2010 by American Society of Hematology

TCR sequences of directly sorted total tetramer+ CD8+ T cells, or tetramer+ cells sorted by effector-memory phenotype and/or CD57 expression. TCR sequences of directly sorted total tetramer+ CD8+ T cells, or tetramer+ cells sorted by effector-memory phenotype and/or CD57 expression. Dirk Meyer-Olson et al. Blood 2010;116:396-405 ©2010 by American Society of Hematology

Selective expansions of distinct HIV-epitope–specific clonotypes into terminally differentiated effector-memory phenotypes (CCR7−/CD45RA+) and association of B27-KK10 tetramer binding with expression of distinct clonotypes and effector-memory differentiation. Selective expansions of distinct HIV-epitope–specific clonotypes into terminally differentiated effector-memory phenotypes (CCR7−/CD45RA+) and association of B27-KK10 tetramer binding with expression of distinct clonotypes and effector-memory differentiation. (A) Analysis of the TRBV populations representing single clonotypes of B8-FL8 tetramer+ cells by flow cytometry. (B) TRBV usage of distinct clonotypes and CCR7/CD45RO expression on B27-KK10 tetramer cells in subject 10022. (C) Pie charts comparing the relative T-cell memory distribution of B8-FL8+ and B27-KK10+ clonotypes identified by TRBV expression in subject 10022. (D) Frequency of clonotypes within the B27-KK10 tetramer+ population in subject 10022. (E) TRBV usage and CCR7/CD45RO expression on B27-KK10 tetramer dim and bright cells in subject 10022. Dirk Meyer-Olson et al. Blood 2010;116:396-405 ©2010 by American Society of Hematology

Differential CD57 expression on HIV-1-tetramer+ CD8+ T-cell memory subsets. Differential CD57 expression on HIV-1-tetramer+ CD8+ T-cell memory subsets. (A) CD57 expression on 2 HIV-epitope specific CD8+ T-cell responses in subject 10001. (B) Lower expression of CD57 on human leukocyte antigen B8-FL8+ TemRA phenotype cells compared with TemRO phenotype cells in subject 10071. (C) Summary of CD57 expression on different memory populations on total CD8+ T cells and HIV-specific CD8+ T cells. (D) Distribution of CD57 on different memory subsets on TRBV+ and TRBV− HIV-specific CD8+ T cells in subject 10071. Dirk Meyer-Olson et al. Blood 2010;116:396-405 ©2010 by American Society of Hematology

Different expression patterns of CD57 and effector-memory marker on HIV-specific clonotypes. Different expression patterns of CD57 and effector-memory marker on HIV-specific clonotypes. Relative distribution of CD57+ (A) and TemRA-phenotype cells (B) between dominant (most frequent) clonotypes and subdominant (less frequent) clonotypes within HIV-epitope–specific CD8+ T-cell responses. (C) Relationship of CD57+ expression on the same clonotype expressing either TemRO or TemRA phenotype marker. Dirk Meyer-Olson et al. Blood 2010;116:396-405 ©2010 by American Society of Hematology