Biomass composition and lignin chemistry of energy sorghum

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Biomass composition and lignin chemistry of energy sorghum BRC Science Highlight Biomass composition and lignin chemistry of energy sorghum Objective Determine the extent and basis of compositional variation of shoot biomass of the energy Sorghum bicolor hybrid TX08001 during development under field conditions. Approach Characterize composition of sorghum biomass. Use near-infrared spectroscopy to analyze internode composition, high-performance liquid chromatography and other analytical methods to quantify soluble carbohydrates, nuclear magnetic resonance spectroscopy to characterize cell wall composition, and Derivatization Followed by Reductive Cleavage to analyze lignin composition. Conduct statistical analyses using the two tailed t-test of mean comparisons. Results/Impacts A panel of diverse energy sorghum genotypes varied ~6-fold in the ratio of stem structural to soluble biomass after 150 days of growth. TX08001 stem sucrose content varied during development, whereas the stem cell wall composition remained constant late in the season. Analysis of irrigated vs. non-irrigated TX08001 revealed that non-irrigated hybrids exhibited a 50% reduction in total cell wall biomass, an ~2-fold increase in stem sugars, and an ~25% increase in water extractives relative to irrigated hybrids. This study provides a baseline of information to help guide further optimization of energy sorghum composition for various end-uses. Notes: text Title again: Text 1-2 sentence summary? Impact of limited irrigation on the biomass and composition of TX08001 stem tissue. (A) The difference in dry biomass of irrigated (IRR) and limited irrigation (L-IRR) stems of TX08001 at 150 days after emergence (DAE). (B) TX08001 stem biomass components as a percentage of the total composition of irrigated and non-irrigated (NIRR) plants at 150 DAE. McKinley, B. A. et al. “Variation in energy sorghum hybrid TX08001 biomass composition and lignin chemistry during development under irrigated and non-irrigated field conditions.” PLOS ONE 13(4), e0195863 (2018) [DOI: 10.1371/journal.pone.0195863]. GLBRC May 2018