Involvement of matrix metalloproteinases in chronic Q fever

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Presentation transcript:

Involvement of matrix metalloproteinases in chronic Q fever A.F.M. Jansen, T. Schoffelen, J. Textoris, J.L. Mege, C.P. Bleeker-Rovers, H.I.J. Roest, P.C. Wever, L.A.B. Joosten, M.G. Netea, E. van de Vosse, M. van Deuren Clinical Microbiology and Infection Volume 23, Issue 7, Pages 487.e7-487.e13 (July 2017) DOI: 10.1016/j.cmi.2017.01.022 Copyright © 2017 European Society of Clinical Microbiology and Infectious Diseases Terms and Conditions

Fig. 1 Matrix metalloproteinase 1 (MMP-1) and MMP-9 production in peripheral blood mononuclear cells (PBMCs). MMP-1 and MMP-9 production after Escherichia coli lipopolysaccharide (LPS) and Coxiella burnetii stimulation in PBMCs. Healthy controls (n=10), individuals with past Q fever (n=10), patients with chronic Q fever (n=10). (a) MMP-1 concentration in PBMCs incubated with either culture medium, E. coli LPS 10 ng/mL or C. burnetii Nine Mile 107/mL. (b) MMP-9 concentration in PBMCs stimulated with either culture medium, E. coli LPS or C. burnetii. Cb Nm 1×107, C. burnetii Nine Mile concentration 1×107/mL. Medians with 1.5 interquartile range are shown. Analysis was performed using the Wilcoxon signed rank test for difference in stimuli between groups, the Kruskal–Wallis test with Dunn’s Multiple Comparison test was used to determine differences within groups. *p<0.05, **p<0.01, *** p<0.001, ns, not significant. Clinical Microbiology and Infection 2017 23, 487.e7-487.e13DOI: (10.1016/j.cmi.2017.01.022) Copyright © 2017 European Society of Clinical Microbiology and Infectious Diseases Terms and Conditions

Fig. 2 Serum concentrations of matrix metalloproteinase 2 (MMP-2), MMP-7 and MMP-10 in control individuals with similar vascular disease (n=10), past Q fever infected individuals with vascular disease (n=10) and patients with chronic Q fever (n=27) serum obtained within 1.5 years after diagnosis. Medians with interquartile range are indicated. *p<0.05; ns, p>0.05. Clinical Microbiology and Infection 2017 23, 487.e7-487.e13DOI: (10.1016/j.cmi.2017.01.022) Copyright © 2017 European Society of Clinical Microbiology and Infectious Diseases Terms and Conditions

Fig. 3 Matrix metalloproteinase 1 (MMP-1 and MMP-9) production in peripheral blood mononuclear cells (PBMCs) pre-incubated with doxycycline. (a) MMP-1 and (b) MMP-9 concentrations in PBMCs (n=9) of healthy blood donors, pre-incubated with doxycycline and stimulated with Coxiella burnetii NM in 107/mL. Medians with interquartile range are provided. Asterisks indicate significant p-values (p<0.05) compared with culture medium (RPMI). *p<0.05. Clinical Microbiology and Infection 2017 23, 487.e7-487.e13DOI: (10.1016/j.cmi.2017.01.022) Copyright © 2017 European Society of Clinical Microbiology and Infectious Diseases Terms and Conditions