Figure 1 Mast Cells per mm2 80 60 40 20 T-blue c-kit Rejecting Naive T-blue c-kit P = 0.001 Mast Cells per mm2 P = 0.003 Rejecting Naive Tolerant Syngeneic Figure 1

Figure 3 Mast Cells per mm2 (Toluidine-blue) Days post TX 120 100 80 60 40 20 P = 0.02 P = 0.1 Mast Cells per mm2 (Toluidine-blue) Naive Tolerant Syngeneic 100 0 7 14 50 100 Days post TX Figure 3

Figure 4 Mast Cells per mm2 (Toluidin-blue) 30 20 10 P = 0.9 P = 0.005 WT C3-/- C3aR-/- P = 0.4 P = 0.004 Mast Cells per mm2 (Toluidin-blue) P = 0.008 dLN LN dLN LN dLN LN Figure 4

A Figure 5 Mast Cells per mm2 (Toluidine-blue) Days post TX 120 100 80 60 40 20 Tolerant Rejecting Syngeneic P = 0.05 Mast Cells per mm2 (Toluidine-blue) P = 0.04 P = 0.9 P = 0.03 7 7 100 7 14 50 100 Days post TX Figure 5

A B Figure 6 Mast Cells per mm2 (Toluidine-blue) 80 60 40 20 WT C3-/- C3aR-/- 80 60 40 20 P = 0.016 P = 0.1 P = 0.029 Mast Cells per mm2 (Toluidine-blue) Mast Cells per mm2 (c-kit) P = 0.016 P = 0.5 P = 0.029 P = 0.9 WT C3-/- C3aR-/- P = 0.2 Naive skin Skin grafts Naive Naive Naive Graft Graft Graft Figure 6