Masui_suppl Fig1 TMab-6 sc-7215 (0.5 μg/mL) (1.0 μg/mL) Case: Astro_2

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Masui_suppl Fig1 TMab-6 sc-7215 (0.5 μg/mL) (1.0 μg/mL) Case: Astro_2 (DA) TERT-wildtype Case: Oligo_1 (AO) TERT-mutant (C228T) TMab-6 (0.5 μg/mL) sc-7215 (1.0 μg/mL) Case: Oligo_10 Case: Astro_4 (AA) Supplementary Figure 1: Immunostaining of TERT protein in human gliomas with TMab-6 and sc-7215. We immunostained TERT wildtype and mutant gliomas with our newly developed TMab-6 and the most widely used, commercially available sc-7215. Both antibodies detected nuclear immunoreactitvity of TERT in TERT wildtype and mutated gliomas although the staining intensities were variable. AA, anaplastic astrocytoma; AO, anaplastic oligodendroglioma. Scale bar = 40 μm. Supplementary Figure 1: Immunostaining of TERT protein in human gliomas with TMab-6 and sc-7215. We immunostained TERT wildtype and mutant gliomas with our newly developed TMab-6 and the most widely used, commercially available sc-7215. Both antibodies detected nuclear immunoreactitvity of TERT in TERT wildtype and mutated gliomas although the staining intensities were variable. AA, anaplastic astrocytoma; AO, anaplastic oligodendroglioma. Scale bar = 40 μm.